This kit uses a colorimetric Loop-Mediated Isothermal Amplification (LAMP)-based solution for rapid dengue virus serotypes 1-4 detection.
Features
Detection is simple and fast colorimetric LAMP reaction by using a RGB Reader¢â device. No other instrument is required for this assay, except for a 65¡É heat source. (Mini-Dry Bath is recommended.)
Utilizes crude nasal swab samples without RNA preparation steps including lysis.
Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the kit. (The inclusion of dUTP and thermolabile UDG in the kit reduces the possibility of carryover contamination, where unintended product of a previous amplification can serve as the substrate of subsequent reaction. Thermolabile UDG is completely inactivated at temperature above 50¡É, thereby having no effect on the amplification reaction.)
End-point colorimetric detection via a metal indicator (hydroxy naphthol blue)
Specimens | Whole blood, serum |
---|---|
Contents | Dengue virus serotypes 1-4 (3' non-coding region) / Amplification control |
Storage | -20¡É (frozen storage) |
Expire date | 6 months (from the date of manufacture) |
Result | Simply confirmed by a RGB Reader¢â device indicating positive/ negative or invalid |
Specimens collection
Virus elution
Virus addition
Amplification
Detection
ÀÌ Å°Æ®´Â Ç÷ûÇü 1-4 ´ó±â ¹ÙÀÌ·¯½ºÀÇ ½Å¼ÓÇÑ °ËÃâÀ» À§ÇÑ ¹ß»ö LAMP(Loop-Mediated Isothermal Amplification) ±â¹ÝÀÇ ¼Ö·ç¼ÇÀ» »ç¿ëÇÕ´Ï´Ù.
°ËÃâÀº RGB Reader¢â ÀåÄ¡¸¦ »ç¿ëÇÏ°í °£´ÜÇÏ°í ºü¸¥ ¹ß»ö LAMP ¹ÝÀÀÀÔ´Ï´Ù. ÀÌ ºÐ¼®¿¡´Â 65¡É ¿¿øÀ» Á¦¿ÜÇϰí´Â ´Ù¸¥ ±â±â°¡ ÇÊ¿äÇÏÁö ¾Ê½À´Ï´Ù. (Mini-Dry Bath¸¦ ±ÇÀåÇÕ´Ï´Ù.)
¿ëÇØ °úÁ¤ µîÀÇ Æ¯º°ÇÑ RNA Áغñ ´Ü°è ¾øÀÌ ¿ø·¡ÀÇ ºñ° ¸éºÀ »ùÇÃÀ» Ȱ¿ëÇÕ´Ï´Ù.
ŰƮ¿¡ Æ÷ÇÔµÈ ³»¿¼º UDG ¹× dUTP·Î ij¸®¿À¹ö ¿À¿° À§ÇèÀ» ÁÙÀÔ´Ï´Ù. (ŰƮ¿¡ dUTP¿Í thermolabile UDG¸¦ Æ÷ÇÔÇϸé ÀÌÀü ÁõÆøÀÇ ÀǵµÇÏÁö ¾ÊÀº »ý¼º¹°ÀÌ ÈÄ¼Ó ¹ÝÀÀÀÇ ±âÁúÀÌ µÉ ¼ö ÀÖ´Â carryover ¿À¿° °¡´É¼ºÀÌ ÁÙ¾îµì´Ï´Ù. Thermolabile UDG´Â 50¡É ÀÌ»óÀÇ ¿Âµµ¿¡¼ ¿ÏÀüÈ÷ ºñȰ¼ºÈµÇ¹Ç·Î ÁõÆø ¹ÝÀÀ¿¡´Â ¿µÇâÀÌ ¾ø½À´Ï´Ù.)
±Ý¼Ó Áö½Ã¾à(ÇÏÀ̵å·Ï½Ã³ªÇÁÅçºí·ç)À» ÅëÇÑ Á¾Á¡ ºñ»ö °ËÃâ
Ç¥º» | Ç÷¾×, Ç÷û |
---|---|
³»¿ë | Dengue virus serotypes 1-4 (3' non-coding region) / Amplification control |
º¸°ü | -20¡É (³Ãµ¿º¸°ü) |
À¯Åë±âÇÑ | 6°³¿ù (Á¦Á¶ÀϷκÎÅÍ) |
°á°ú | ¾ç¼º/À½¼º ¶Ç´Â ¹«È¿¸¦ ³ªÅ¸³»´Â RGB Reader¢â ÀåÄ¡·Î °£´ÜÇÏ°Ô È®ÀÎ |
Specimens collection
Virus elution
Virus addition
Amplification
Detection