Protein Purification Protein Purification Protein Purification

Protein Purification

´Ü¹éÁúÀÇ ¼ø¼öÇÑ ºÐ¸®´Â ÀϹÝÀûÀÌÁö¸¸ ½Ã°£ÀÌ ¸¹ÀÌ °É¸®´Â ÀÛ¾÷ÀÔ´Ï´Ù.
New England Biolabs´Â ´Ü¹éÁúÀÇ ½Å¼ÓÇÑ ºÐ¸® ¹× Á¤Á¦¸¦ À§ÇØ »ç¿ëÇϱ⠽±°í ±¸Ã¼ÀûÀ̸ç, ¿©·¯ °¡Áö Çü½ÄÀ¸·Î ´Ù¾çÇÑ resin ¹× magnetic beads(ÀÚ¼ºÃ¼ ºñµå)¸¦ Á¦°øÇÕ´Ï´Ù. NEBÀÇ magnetic beads´Â high-throughput ´Ü¹éÁú ½ºÅ©¸®´× ¹× ¼Ò±Ô¸ð ´Ü¹éÁú ºÐ¸®¿Í °ü·ÃµÈ ÀÀ¿ë ºÐ¾ß¿¡ ¸Å¿ì ÀÌ»óÀûÀÔ´Ï´Ù. ¸¶±×³×ƽ ºñµå¸¦ »ç¿ëÇϸé taggingµÈ ´Ü¹éÁú ¹× Ç×üÀÇ Ä£È­¼º Á¤Á¦¸¦ Æí¸®ÇÏ°í ºü¸£°Ô ¼öÇàÇÒ ¼ö ÀÖ½À´Ï´Ù. NEBÀÇ resinsÀº tagged ´Ü¹éÁú¿¡ ´ëÇÑ °£´ÜÇÑ ¿ø½ºÅÜ Á¤Á¦ Àü·«À» °¡´ÉÇÏ°Ô ÇÏ¿© °í¼øµµ ´Ü¹éÁúÀÇ ³ôÀº ¼öÀ²À» Á¦°øÇÕ´Ï´Ù.

Protein Purification includes these subcategories

NEBÀ̹ÌÁö ProteinPurification1

Cell-free E.coli Protein Synthesis System (NEB #E5360)

E. coli¿¡¼­ ÃßÃâµÈ T7 RNA Polymerase¸¦ ÀÌ¿ëÇÏ¿© 17~230kDa Å©±âÀÇ ´Ü¹éÁúÀ» ºü¸¥ ½Ã°£³»¿¡ ÇÕ¼ºÇÒ ¼ö ÀÖ¾î °£ÆíÇÏ°í ºü¸£°Ô Cell »ç¿ë¾øÀÌ ´Ü¹éÁúÀ» ÇÕ¼ºÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÔ´Ï´Ù. .....Read more

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NEBÀ̹ÌÁö ProteinPurification2

NEBExpress¢ç E. coli Lysis Reagent (NEB #P8116)

E. coli¿¡¼­ °£´ÜÈ÷ Soluble proteinÀ» ÃßÃâÇÒ ¼ö ÀÖ´Â Lysis Reagents·Î ³ôÀº È°¼º, º¯¼º ¾ø´Â ´Ü¹éÁúÀ» ¾òÀ» ¼ö ÀÖ½À´Ï´Ù. .....Read more

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NEB À̹ÌÁö

NEBExpress¢â Ni-NTA Magnetic Beads (NEB #S1423)

Agarose ±â¹ÝÀÇ ÃÊ»óÀÚ¼º ¸¶ÀÌÅ©·ÎÀÔÀÚ·Î ºñƯÀÌÀû °áÇÕÀÌ ¸Å¿ì ³·¾Æ °íÁ¤µÈ ´Ü¹éÁúÀÇ ÈÄ¼Ó ½ÇÇè Àû¿ëÀÌ °¡´ÉÄÉ ÇÏ´Â Ni-NTA Magnetic Beads·Î native ¹× denaturing condition¿¡¼­ ºÐ¸® Á¤Á¦ ÇÒ ¼ö ÀÖ½À´Ï´Ù. .....Read more

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NEB À̹ÌÁö

IMPACT¢â Kit (NEB #E6901)

IMPACT¢â Kit ½Ã½ºÅÛÀº Protein splicing elements (Inteins)ÀÇ self-cleavage activity¸¦ È°¿ëÇÏ¿© ģȭ¼º ű׿¡¼­ Ç¥Àû ´Ü¹éÁúÀ» ºÐ¸®ÇÏ´Â »õ·Î¿î ´Ü¹éÁú Á¤Á¦ ¹æ¹ýÀÔ´Ï´Ù. Affinity tag¸¦ protease·Î Á¦°ÅÇÒ ÇÊ¿ä°¡ ¾ø¾î¼­ ´ÜÀÏ Ä®·³À¸·Î Á¤Á¦, Vector derived amino acids ¾øÀÌ, Ÿ°Ù ´Ü¹éÁú ȹµæ, N-terminal methionine residue ¾øÀÌ ´Ü¹éÁú Á¤Á¦ °¡´ÉÇÏ°í T7 promoter-driven systemÀ¸·Î ³ôÀº ¹ßÇöÀÌ Æ¯Â¡ÀÔ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ