LunaScript¢ç Multiplex One-Step RT-PCR Kit (NEB #E1555)
NEB LunaScript¢ç·Î RNA ¿¡¼ RT-PCR ±îÁö Çѹø¿¡, Multiplex ±îÁö ÇÒ ¼ö ÀÖ½À´Ï´Ù. .....Read more
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NEB´Â ±¤¹üÀ§ÇÑ DNA ÁßÇÕÈ¿¼Ò¸¦ Á¦°øÇϸç DNA ÁõÆø ¹× PCRÀ» À§ÇÑ Çõ½ÅÀûÀÌ°í °íÇ°ÁúÀ» º¸ÀåÇÏ´Â µµ±¸µéÀ» °³¹ß ÇÏ¿´½À´Ï´Ù.
NEB´Â ´ÙÀ½À» Æ÷ÇÔÇÏ¿© ´Ù¾çÇÑ DNA ¹× RNA ÁßÇÕÈ¿¼Ò, nucleotide mixes, Å°Æ®, Master mix µîÀ» Á¦°øÇÕ´Ï´Ù.
¡¤ Luna¢ç qPCR and RT-qPCR Reagents :
for rapid, sensitive and precise detection of RNA, DNA and cDNA targets
¡¤ Q5¢ç High-Fidelity DNA Polymerase : for robust ultra high-fidelity PCR
¡¤
OneTaq ¢ç : for robust routine PCR
¡¤ Phusion¢ç : for high-fidelity PCR
NEB LunaScript¢ç·Î RNA ¿¡¼ RT-PCR ±îÁö Çѹø¿¡, Multiplex ±îÁö ÇÒ ¼ö ÀÖ½À´Ï´Ù. .....Read more
Single tube ·Î Á¦°øµÇ´Â one-step-RT-qPCR master mix·Î 5 Á¾·ù target À¸·Î multiplex qPCR ÀÌ °¡´ÉÇÏ°í, UDG ¿Í dUTP ¸¦ Æ÷ÇÔÇÏ¿© ±³Â÷ ¿À¿°±îÁö °¨¼Ò ½ÃÄ×½À´Ï´Ù. ROX dye ¸¦ Æ÷ÇÔ ÇÑ M0319 ¿Í Æ÷ÇÔÇÏÁö ¾ÊÀº M3029 °¡ ÀÖ½À´Ï´Ù. .....Read more
Lyophilization Áï, µ¿°á°ÇÁ¶¶õ Àú¿Â¿¡¼ ±Þ¼Ó °ÇÁ¶·Î ¿À·¡ÀüºÎÅÍ ¹ßÀü½ÃÄÑ¿Â ¹æ¹ýÀ¸·Î ÀÌ¹Ì ¸¹Àº Á¦Ç°µéÀÌ º¸°ü°ú Àü´Þ, ºñ¿ëÀý°¨ µîÀ» À§ÇØ ÀÌ¿ëÇÏ°í ÀÖ½À´Ï´Ù. NEB ÀÇ LypPrime Luna Probe One-Step-RT-qPCR Mix´Â Áø´Ü¿¡ ¿ëÀÌ, ½Ç¿Â º¸°ü, ÇöÀå¿¡¼ »ç¿ëÀÌ ¿ëÀÌ ÇÕ´Ï´Ù. µ¿°á °ÇÁ¶ÇüÅ·Π»ó¿Âº¸°ü 2³â±îÁö °¡´ÉÇÑ LyoPrime Luna Probe One-Step-RT-qPCR Mix ´Â UDG ¸¦ Æ÷ÇÔ ÇÏ°í ÀÖÀ¸¸ç, 5°³ÀÇ targets ÀÇ multiplex qPCR ÀÌ °¡´ÉÇÕ´Ï´Ù. ÷°¡ÇÏ´Â nuclease free water ÀÇ ¾ç¿¡ µû¶ó 2X ¿¡¼ 4X ±îÁö »ç¿ëÀÚÀÇ ¿ä±¸¿¡ ¸ÂÃç »ç¿ëµÉ ¼ö ÀÖ½À´Ï´Ù. ½Ç¿Â¿¡¼ ¾ÈÀüÇÏ°Ô ¹ÝÀÀ set- up À» ÇÒ ¼ö ÀÖ°í, ¼º´ÉÀº liquid type ÀÇ Luna RT-qPCR mixture ¿Í µ¿ÀÏÇÕ´Ï´Ù. OTC ±¸¸Å°¡ °¡´ÉÇÏ¿© ¿øÇÏ´Â ºÐ·® ¸¸Å ´ë·®À¸·Îµµ ±¸¸Å°¡ °¡´ÉÇÕ´Ï´Ù. .....Read more
Ç÷¾×»ùÇÿ¡¼ DNA ÃßÃâ Á¤Á¦ ¾øÀÌ ¹Ù·Î PCR À» ¿øÇϽŴٸé Q5 Blood Direct 2X Master Mix ! Whole blood cells, dry blot spots¿¡¼ DNA ÃßÃâ °úÁ¤ÀÌ ÇÊ¿ä¾ø´Â ¿¬±¸ÀÚ Ä£ÈÀûÀÎ Á¦Ç°ÀÔ´Ï´Ù. .....Read more
NEB LunaScript´Â 55µµ½Ã ³ôÀº ¹ÝÀÀ¿Âµµ¿¡¼ 13ºÐ ³»¿¡ cDNA ÇÕ¼ºÀ» ÇÕ´Ï´Ù.
´Ù¾çÇÑ polymerase ³ª PCR, qPCR ¿¡ ÇÊ¿äÇÑ enzyme, kit Á¤º¸°¡ ÇÊ¿ä ÇÏ½Ã¸é ¹®ÀÇ ÁÖ¼¼¿ä
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¹è¾çÇÑ ¼¼Æ÷¿¡¼ RNA Á¤·®È±îÁö One-StepÀ¸·Î!
¤ý100 lysis preps ¹× 500 one-step RT-qPCR ¹ÝÀÀ ½Ã¾à (Probe)
¤ý´Ù¾çÇÑ ¼¼Æ÷ÁÖ Àü¹Ý¿¡¼ÀÇ È¿°úÀû ¼¼Æ÷ ºÐÇØ (¼¼Æ÷¼ö 10~100,000°³)
¤ý15ºÐ³» cell lysis, RNA release ¹× genomic DNA Á¦°Å °¡´É
¤ýLuna¢ç WarmStart¢ç RT Enzyme Mix¸¦ ÅëÇÑ ¿¾ÈÁ¤¼º ¹× ½Ç¿Â ¼³Á¤ ±â´É Çâ»ó
¤ýLuna Cell Ready Lysis Module (NEB #E3032 Ãß°¡ÀûÀ¸·Î º°µµ±¸¸Å °¡´É)
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phi29-XT RCA Kit¿¡´Â ¿øÇü ½ÃÄö½ºÀÇ ±æ°í ¹Ýº¹ÀûÀÎ º¹Á¦º»À» »ý¼ºÇÏ¿© ¿øÇü DNA¸¦ Áö¼ÓÀûÀ¸·Î ÁõÆøÇÏ´Â µî¿Â ÁõÆø ¹æ¹ýÀ» »ç¿ëÇÑ ¹Î°¨ÇÏ°í °ß°íÇÑ ·Ñ¸µ ¼Å¬ ÁõÆø(RCA)¿¡ ÇÊ¿äÇÑ ¸ðµç °ÍÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
¤ý ¿ ¾ÈÁ¤¼º°ú °¨µµ°¡ °³¼± ¼³°èµÈ ÁßÇÕÈ¿¼Ò·Î phi29-XT DNA ÁßÇÕÈ¿¼Ò´Â wild-type phi29 DNA ÁßÇÕÈ¿¼Òº¸´Ù ªÀº ¹ÝÀÀ ½Ã°£¿¡ ´õ ³ôÀº ¼öÀ²·Î »ý¼º
¤ý ÃÖ¼Ò 1fgÀÇ ¿øÇü DNA¿¡¼µµ ÁõÆø
¤ý ÀϹÝÀûÀÎ ¿øÇü DNA ¼¿À» ÁõÆøÇϱâ À§ÇÑ dNTP ¹× exonuclease-resistant random primers (phosphorothioate °áÇÕ)Æ÷ÇÔ
¤ý Á¤Á¦µÈ ¿øÇü DNA(´ÜÀÏ ¶Ç´Â ÀÌÁß °¡´Ú)À̰ųª DNA ÃßÃâ ¾øÀÌ ¾×ü ¹èÁö ¹è¾ç, Agar plate colony ¹× ±Û¸®¼¼·Ñ ½ºÅåÀ» ¹Ù·Î »ç¿ë °¡´É
¤ý RCA Á¦Ç°Àº DNA ½ÃÄö½Ì, cell-free DNA ³óÃà, cell-free ´Ü¹éÁú ¹ßÇö ¹× DNA ¹ÙÀÌ¿À¼¾¼¿Í °°Àº ´Ù¿î½ºÆ®¸² ÀÀ¿ë ºÐ¾ß¿¡¼ Á÷Á¢ »ç¿ë °¡´É
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µî¿ÂÇÙ»êÁõÆø(LAMP)À» À§ÇÑ ÃÖ°íÀÇ ¼±Åà ! NEB Bst 2.0 WarmStart¢ç DNA Polymerase¿Í ÇÔ²² Çϼ¼¿ä.
¤ý Isothermal amplification(LAMP)¿¡ ÃÖÀûÈ
¤ý Contains 5' ¡æ 3' DNA polymerase activity and strong strand - displacement activity, lacks 5' ¡æ 3' exonuclease activity.
¤ý WarmStart : 45¡ÉÀÌÇÏ¿¡¼ ¹ÝÀÀ ¾ïÁ¦ (½Ç¿Â¿¡¼ ¹ÝÀÀ setup). off - target ÁõÆø Á¦°Å, ¹ÝÀÀ È¿À² Áõ°¡
¤ý ³ôÀº salt tolerance : ~120 mM monovalent salt.
¤ý Optimal reaction performance : 60 - 72¡É
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NEBÀÇ Colorimetric LAMP Á¦Ç°Àº ºÐÀÚ ÇöÀåÁø´Ü (POCT)¿¡ ÃÖÀûÈµÈ Á¦Ç°ÀÔ´Ï´Ù.
" Fast, No thermal cycler, No fluorescent detector "
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OneTaq¢ç DNA Polymerase
¤ý 2X higher fidelity than Taq [ ³ôÀº Á¤È®¼º ]
¤ý Ideal for routine, QT- or GC-rich templates [ ´Ù¾çÇÑ ¹ü¿ë¼º ]
¤ý Hot start and master mix versions available [ ¼±Åà °¡´É ]
OneTaq¢ç Hot Start DNA Polymerase
¤ý Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step [ º°µµ ´Ü°è°¡ ¾ø´Â °£Æí¼º ]
¤ý Robust yield with minimal optimization [ ÃÖÀûÈ ]
¤ý Ideal for routine, QT- or GC-rich templates [ ´Ù¾çÇÑ ¹ü¿ë¼º ]
¤ý Room temperature reaction setup [ ½Ç¿Â ¹ÝÀÀ ]
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Thermostable RNase H´Â ¿¾ÈÁ¤¼º°ú ÇÔ²² RNA-DNA ÇÏÀ̺긮µåÀÇ RNA °¡´ÚÀ» ƯÀÌÀûÀ¸·Î ºÐÇØÇÏ´Â È¿¼ÒÀÔ´Ï´Ù. .....Read more
PCR ÁõÆøÀ» À§ÇØ Çõ½ÅÀûÀÌ°í ³ôÀº QualityÀÎ NEB Á¦Ç° Solution
¤ý High-Fidelity DNA polymerase and Master Mix
¤ý dNTP Solution mix and Set
¤ý qPCR and RT-qPCR Master Mix and Kit
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¿øÇü DNA ÁõÆøÀ»À§ÇÑ rolling circle amplification(RCA)Àº
¤ý Fast (2h)
¤ý Sensitive (~1fg DNA)
¤ý High Yield (10§¶¡è)
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³ôÀº ¿¾ÈÁ¤¼º, ºü¸¥ ÁøÇ༺ ¹× ¹ÝÀÀ ¿À¿°¹°Áú¿¡ ´ëÇÑ ³»¼ºÀ» ³ªÅ¸³»´Â Group II intron-encoded RTÀÇ Induro¢â Reverse Transcriptase ¸¦ ¼Ò°³ÇÕ´Ï´Ù.
¤ý 󸮼ºÀÌ ¶Ù¾î³ RT(Reverse Transcriptase)´Â ³ôÀº ¼öÀ²ÀÇ ±ä cDNA·Î ºü¸¥ ¹ÝÀÀ °¡´É
¤ý °·ÂÇÑ imhibitor ³»¼ºÀ¸·Î °·ÂÇÑ cDNA ÇÕ¼º ¼º´É °¡´É
¤ý Á÷Á¢ RNA ½ÃÄö½Ì ¹× long read cDNA ½ÃÄö½Ì ¿öÅ©Ç÷οì Áö¿ø
¤ý °í¿Â¿¡¼ °·ÂÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ Çâ»óµÈ ³»¿¼º
¤ý ·¹Æ®·Î¹ÙÀÌ·¯½º RT(Reverse Transcriptase) ¿¡ ÇÊÀûÇÏ´Â Ãæ½Çµµ
.....Read more