NEBExpress¢ç Cell-free E. coli Protein Synthesis System
T7 RNA Polymerase¸¦ ÅëÇØ 2-4½Ã°£µ¿¾È 17 – 230kDa±îÁöÀÇ ´Ü¹éÁúÀÌ ÇÕ¼ºµÇ¸ç,
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´ë½Å, ´Ù¾çÇÑ ¹ßÇö toolÀ» ã¾Æº¸°í ´Ù¾çÇÑ Á¢±Ù ¹æ½ÄÀ» Ž»öÇϸé [Protein Expression at NEB]
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Protein Expression includes these subcategories
T7 RNA Polymerase¸¦ ÅëÇØ 2-4½Ã°£µ¿¾È 17 – 230kDa±îÁöÀÇ ´Ü¹éÁúÀÌ ÇÕ¼ºµÇ¸ç,
ÇÕ¼ºµÈ ´Ü¹éÁúÀº SDS-PAGE, western blot¿¡ ¹Ù·Î »ç¿ë °¡´ÉÇÕ´Ï´Ù.
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NEBExpress¢ç Á¤Á¦ ½Ã½ºÅÛÀ» È°¿ëÇÑ È÷½ºÆ¼µò ÅÂ±ë ´Ü¹éÁú Á¤Á¦ ½Ã½ºÅÛ
NEB¿Í ÇÔ²² Poly-His tagged recombinant protein Á¤Á¦¸¦ °£´ÜÇÏ°Ô !!!
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°·ÂÇÑ Ptac ÇÁ·Î¸ðÅÍ¿Í Maltose °áÇÕ ´Ü¹éÁú(MBP) Ư¡À» È°¿ëÇÏ¿© E. coli ¿¡¼ ¿øÇÏ´Â ´Ü¹éÁúÀÇ solubility¿Í Expression levelÀ» Çâ»ó½ÃÄÑ »ý¼ºµÈ ´Ü¹éÁúÀº Affinity chromatography Á¤Á¦·Î MBP fusion protein purification system ÀÔ´Ï´Ù. .....Read more
´Ü¹éÁú ÇÕ¼ºÇϼ¼¿ä? Çʼö Template¸¸ ÀÖÀ¸¸é µË´Ï´Ù.
¤ýHigh throughput screening ±×¸®°í liquid handling
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