DNA Modifying Enzymes DNA Modifying Enzymes DNA Modifying Enzymes

DNA Modifying Enzymes

ºÐÀÚ»ý¹°ÇÐÀÇ ¹ßÀüÀº ÀÌ ºÐ¾ßÀÇ ÇÙ½ÉÀÎ µµ±¸¿Í ±â¼úÀÇ Áö¼ÓÀûÀÎ Çõ½Å°ú ÀüÁøÀ» ÇÊ¿ä·Î ÇÕ´Ï´Ù. New England Biolabs´Â DNA ±â¹Ý Á¶ÀÛÀÇ ÃÖ÷´Ü¿µ¿ªÀ» ¹ßÀü½ÃŰ´Â µ¥ µµ¿òÀÌ µÇ´Â ´Ù¾çÇÑ Çʼö ½Ã¾àÀ» Á¦°øÇϱâ À§ÇØ ÃÖ¼±À» ´ÙÇϰí ÀÖ½À´Ï´Ù. NEBÀÇ È¿¼ÒÇÐ Àü¹® Áö½ÄÀº °æÀï»ç¿Í Â÷º°È­µÇ¾î ´ÙÀ½À» Á¦°øÇÏ´Â ºÐÀÚ »ý¹°ÇÐÀ» À§ÇÑ È¿¼Ò¸¦ »ý»êÇÒ ¼ö ÀÖ°Ô ÇÕ´Ï´Ù. 250°³ ÀÌ»óÀÇ ÀçÁ¶ÇÕ È¿¼Ò¸¦ Æ÷ÇÔÇÑ NEBÀÇ °í¼øµµ enzyme Àº Ź¿ùÇÑ ¼º´É°ú °¡Ä¡¸¦ Á¦°øÇÕ´Ï´Ù.

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T4 DNA Ligase (NEB #M0202)

duplex DNA, RNA ¿¡ ÀÖ´Â 5¡¯-Àλê±â ¸»´Ü°ú 3¡¯- ¼ö»ê±â ¸»´Ü ºÎÀ§ »çÀÌ¿¡ phosphodiester bond ¸¦ Çü¼ºÇÕ´Ï´Ù. Immobilized T4 DNA Ligase(NEB #M0569S) ´Â T4 DNA Ligase·Î ÄÚÆÃµÈ ÀÚ¼º ºñµåÀÇ ÇöŹ¾× ¹ÝÀÀ ÈÄ¿¡ ÀÚ¼ºÀ¸·Î È¿¼Ò¸¦ Á¦°ÅÇÑ ÈÄ ¿øÇÏ´Â ligate ÃëÇϰí È¿¼Ò´Â Àç»ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù. .....Read more

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NEBÀ̹ÌÁö DNAModifyingEnzymes2

Ligase Master Mix (NEB #M1100S)

High efficiency & high-fidelityÀ» ÀÚ¶ûÇϰí NEB Type IIS restriction enzymes°ú Golden Gate Assembly¿¡ Àû¿ë °¡´ÉÇÕ´Ï´Ù. T4 DNA Ligase¿Í Àü¿ë Ligation enhancer°¡ Æ÷ÇÔµÈ optimized reaction buffer·Î ±¸¼º µÇ¾î ÀÖ¾î ¼±ÅÃÇÑ NEB Type IIS Á¦ÇÑÈ¿¼Ò¿Í DNA substrates¸¸ ÷°¡ÇÏ¿© Assembly ¼öÇà ÇÒ ¼ö ÀÖ½À´Ï´Ù. .....Read more

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Mismatch Endonuclease I (NEB #M0678)

DNA ¿£µµ´ºÅ¬·¹¾ÆÁ¦·Î ÀϺΠDNA ºÒÀÏÄ¡(T:T, G:G ¹× G:T) ºÎºÐÀ» ÀνÄÇÏ¿© Àý´ÜÀ» Ã˸ÅÇÕ´Ï´Ù. 3'-OH ¹× 5'-phosphodiester bond°¡ ÀÖ´Â 5bp ¿À¹öÇàÀ» ³²±â´Â µÎ °¡´ÚÀÇ ºÒÀÏÄ¡ ¿°±âÀÇ 5' ÂÊ¿¡ ÀÖ´Â 3¹øÂ° phosphodiester °áÇÕÀ» Àý´ÜÇÕ´Ï´Ù. .....Read more

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Msz Exonuclease I (NEB #N0527S)

DNA ƯÀÌÀû ¿¢¼Ò´ºÅ¬·¹¾ÆÁ¦·Î 80¡ÆC¿¡¼­ 1ºÐ¾È¿¡ ¿­¿¡ ÀÇÇÑ ºÒȰ¼ºÈ­°¡ °¡´É ÇÕ´Ï´Ù. Msz Exonuclease I(MszExoI)´Â 45¡ÆC ¹× 60¡ÆC ³»¿¡¼­ È£¿­¼º ½ÇÇè´Ü°è ÀÀ¿ë ºÐ¾ß¿¡¼­ ¼±Çü ´ÜÀÏ °¡´Ú DNA ¿Ã¸®°í Á¦°Å ÇÏ´Â µ¥ ÀÌ¿ëµË´Ï´Ù. 3'¿¡¼­ 5' ¹æÇâÀ¸·Î ¼±Çü ´ÜÀÏ °¡´Ú DNA¿¡¼­ ´ºÅ¬·¹¿ÀƼµå Á¦°Å¸¦ Ã˸ÅÇÏ´Â DNA ƯÀÌÀû ¿¢¼Ò´ºÅ¬·¹¾ÆÁ¦ÀÔ´Ï´Ù. .....Read more

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Thermostable OGG (NEB #M0464S)

¿­¿¡ ¾ÈÁ¤ÀûÀÎ Thermostable oxoguanine glycosylase·Î DNA N-glycosylase ¿Í AP lyase µÎ°¡Áö ±â´ÉÀ» °®°í ÀÖ½À´Ï´Ù. ´Ù¸¥ DNA glycosylases¿Í´Â ´Ù¸£°Ô Thermostable OGG´Â Ưº°È÷ 8-oxoG¸¸ ÀνÄÇϰí Àý´ÜÇÏ¸ç ´Ù¸¥ º¯ÇüµÈ ¿°±â´Â Àý´ÜÇÏÁö ¾Ê½À´Ï´Ù. 25-80¡ÆC »çÀÌÀÇ ¿Âµµ¿¡¼­ ÀÛµ¿, ÃÖÀû¿Âµµ´Â 65-75¡ÆC ÀÔ´Ï´Ù. .....Read more

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