DNA Modifying Enzymes DNA Modifying Enzymes DNA Modifying Enzymes

DNA Modifying Enzymes

ºÐÀÚ»ý¹°ÇÐÀÇ ¹ßÀüÀº ÀÌ ºÐ¾ßÀÇ ÇÙ½ÉÀÎ µµ±¸¿Í ±â¼úÀÇ Áö¼ÓÀûÀÎ Çõ½Å°ú ÀüÁøÀ» ÇÊ¿ä·Î ÇÕ´Ï´Ù. New England Biolabs´Â DNA ±â¹Ý Á¶ÀÛÀÇ ÃÖ÷´Ü¿µ¿ªÀ» ¹ßÀü½ÃÅ°´Â µ¥ µµ¿òÀÌ µÇ´Â ´Ù¾çÇÑ Çʼö ½Ã¾àÀ» Á¦°øÇϱâ À§ÇØ ÃÖ¼±À» ´ÙÇÏ°í ÀÖ½À´Ï´Ù. NEBÀÇ È¿¼ÒÇÐ Àü¹® Áö½ÄÀº °æÀï»ç¿Í Â÷º°È­µÇ¾î ´ÙÀ½À» Á¦°øÇÏ´Â ºÐÀÚ »ý¹°ÇÐÀ» À§ÇÑ È¿¼Ò¸¦ »ý»êÇÒ ¼ö ÀÖ°Ô ÇÕ´Ï´Ù. 250°³ ÀÌ»óÀÇ ÀçÁ¶ÇÕ È¿¼Ò¸¦ Æ÷ÇÔÇÑ NEBÀÇ °í¼øµµ enzyme Àº Ź¿ùÇÑ ¼º´É°ú °¡Ä¡¸¦ Á¦°øÇÕ´Ï´Ù.

NEBÀ̹ÌÁö DNAModifyingEnzymes1

T4 DNA Ligase (NEB #M0202)

duplex DNA, RNA ¿¡ ÀÖ´Â 5¡¯-Àλê±â ¸»´Ü°ú 3¡¯- ¼ö»ê±â ¸»´Ü ºÎÀ§ »çÀÌ¿¡ phosphodiester bond ¸¦ Çü¼ºÇÕ´Ï´Ù. Immobilized T4 DNA Ligase(NEB #M0569S) ´Â T4 DNA Ligase·Î ÄÚÆÃµÈ ÀÚ¼º ºñµåÀÇ ÇöŹ¾× ¹ÝÀÀ ÈÄ¿¡ ÀÚ¼ºÀ¸·Î È¿¼Ò¸¦ Á¦°ÅÇÑ ÈÄ ¿øÇÏ´Â ligate ÃëÇÏ°í È¿¼Ò´Â Àç»ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes2

Ligase Master Mix (NEB #M1100S)

High efficiency & high-fidelityÀ» ÀÚ¶ûÇÏ°í NEB Type IIS restriction enzymes°ú Golden Gate Assembly¿¡ Àû¿ë °¡´ÉÇÕ´Ï´Ù. T4 DNA Ligase¿Í Àü¿ë Ligation enhancer°¡ Æ÷ÇÔµÈ optimized reaction buffer·Î ±¸¼º µÇ¾î ÀÖ¾î ¼±ÅÃÇÑ NEB Type IIS Á¦ÇÑÈ¿¼Ò¿Í DNA substrates¸¸ ÷°¡ÇÏ¿© Assembly ¼öÇà ÇÒ ¼ö ÀÖ½À´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes3

Mismatch Endonuclease I (NEB #M0678)

DNA ¿£µµ´ºÅ¬·¹¾ÆÁ¦·Î ÀϺΠDNA ºÒÀÏÄ¡(T:T, G:G ¹× G:T) ºÎºÐÀ» ÀνÄÇÏ¿© Àý´ÜÀ» Ã˸ÅÇÕ´Ï´Ù. 3'-OH ¹× 5'-phosphodiester bond°¡ ÀÖ´Â 5bp ¿À¹öÇàÀ» ³²±â´Â µÎ °¡´ÚÀÇ ºÒÀÏÄ¡ ¿°±âÀÇ 5' ÂÊ¿¡ ÀÖ´Â 3¹ø° phosphodiester °áÇÕÀ» Àý´ÜÇÕ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes4

Msz Exonuclease I (NEB #N0527S)

DNA ƯÀÌÀû ¿¢¼Ò´ºÅ¬·¹¾ÆÁ¦·Î 80¡ÆC¿¡¼­ 1ºÐ¾È¿¡ ¿­¿¡ ÀÇÇÑ ºÒÈ°¼ºÈ­°¡ °¡´É ÇÕ´Ï´Ù. Msz Exonuclease I(MszExoI)´Â 45¡ÆC ¹× 60¡ÆC ³»¿¡¼­ È£¿­¼º ½ÇÇè´Ü°è ÀÀ¿ë ºÐ¾ß¿¡¼­ ¼±Çü ´ÜÀÏ °¡´Ú DNA ¿Ã¸®°í Á¦°Å ÇÏ´Â µ¥ ÀÌ¿ëµË´Ï´Ù. 3'¿¡¼­ 5' ¹æÇâÀ¸·Î ¼±Çü ´ÜÀÏ °¡´Ú DNA¿¡¼­ ´ºÅ¬·¹¿ÀƼµå Á¦°Å¸¦ Ã˸ÅÇÏ´Â DNA ƯÀÌÀû ¿¢¼Ò´ºÅ¬·¹¾ÆÁ¦ÀÔ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes5

Thermostable OGG (NEB #M0464S)

¿­¿¡ ¾ÈÁ¤ÀûÀÎ Thermostable oxoguanine glycosylase·Î DNA N-glycosylase ¿Í AP lyase µÎ°¡Áö ±â´ÉÀ» °®°í ÀÖ½À´Ï´Ù. ´Ù¸¥ DNA glycosylases¿Í´Â ´Ù¸£°Ô Thermostable OGG´Â Ưº°È÷ 8-oxoG¸¸ ÀνÄÇÏ°í Àý´ÜÇÏ¸ç ´Ù¸¥ º¯ÇüµÈ ¿°±â´Â Àý´ÜÇÏÁö ¾Ê½À´Ï´Ù. 25-80¡ÆC »çÀÌÀÇ ¿Âµµ¿¡¼­ ÀÛµ¿, ÃÖÀû¿Âµµ´Â 65-75¡ÆC ÀÔ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes6

USER¢ç(Uracil-Specific Excision Reagent) Enzymes

UDG´Â Uracil ¿°±âÀÇ Àý´ÜÀ» Ã˸ÅÇÏ¿© phosphodiester backboneÀº ±×´ë·Î µÎ°í ¹«¿°±â(apyrimidinic) ºÎÀ§¸¦ Çü¼ºÇÕ´Ï´Ù. EndonucleaseÀÇ lyase È°¼ºÀº ¹«¿°±â ºÎÀ§ÀÇ 3' ¹× 5' phosphodiester backboneÀ» Æı«ÇÏ¿© ¿°±â°¡ ¾ø´Â deoxyribose¸¦ ¹æÃâÇÕ´Ï´Ù. USER(Uracil-Specific Excision Reagent) È¿¼Ò´Â ÀÌ µÎ°¡Áö È¿¼Ò È°µ¿À» °áÇÕÇÏ¿© uracil residue À§Ä¡¿¡¼­ ´ÜÀÏ nucleotide gapÀ» »ý¼ºÇÕ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes7

DNase I-XT

°æÀïÁ¦Ç°(TURBO DNase) ´ëºñ °¡°Ý°ú ¼º´ÉÀÌ ¿ì¼öÇÑ NEBÀÇ ½ÅÁ¦Ç°À» ¼Ò°³ÇÕ´Ï´Ù

¤ý IVT ¹× RNA preps°ú °°ÀÌ ´õ ¸¹Àº ¾çÀÇ ¿°À» Æ÷ÇÔÇÏ´Â ¹ÝÀÀ¿¡ »ç¿ë
¤ý Èñ¼®ÇÒ ÇÊ¿ä ¾øÀÌ IVT ¹ÝÀÀ¿¡ Á÷Á¢ Ãß°¡
¤ý IVT ¹ÝÀÀ ¹× RNA Áغñ¿¡¼­ DNA¸¦ È¿À²ÀûÀ¸·Î Á¦°Å
¤ý RNase-free È¿¼Ò´Â ±¤¹üÀ§ÇÑ ¿° Á¶°Ç (ÃÖ´ë 300mM)À» °ßµö´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes8

ET SSB

PCR È¿À²ÀÌ ³·¾Æ °í¹ÎÀ̽źÐ? NEB»ç°¡ Á¦°øÇÏ´Â ET SSB¸¦ »ç¿ëÇÏ½Ã¸é °í¹Î ÇØ°á!
ET-SSB [ Extreme Thermostable Single-Stranded DNA Binding Protein ]


´ÜÀÏ°¡´Ú DNA¿¡ ³ôÀº ģȭµµ·Î °áÇÕÇϸç, ÀÌÁß³ª¼±À» ºÒ¾ÈÁ¤È­ÇÏ¿© DNA ÁßÇÕÈ¿¼Ò ¹ÝÀÀ Çâ»óÀ» µµ¿ÍÁÖ´Â ´Ü¹éÁúÀÔ´Ï´Ù. ¶ÇÇÑ ¿­¿¡ °­ÇØ 95¡É¿¡¼­µµ È°¼ºÀ» À¯ÁöÇÕ´Ï´Ù. .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes9

Phosphatase

Phosphatase¸¦ ã°í ÀÖ½À´Ï±î? NEBÀÇ Phosphatase¸¦ ÃßõÇÕ´Ï´Ù.
´Ü¹éÁúÀÇ Phosphorylation / DephosphorylationÀº ¼¼Æ÷ÀÇ ½ÅÈ£ Àü´Þ °úÁ¤¿¡¼­ ÀϾ´Â Çʼö Çö»óÀÔ´Ï´Ù. ±×¸®°í ¼¼Æ÷ÀÇ ½ÅÈ£ Àü´Þ °úÁ¤À̳ª ±â´ÉÀ» ¿¬±¸ÇÏ´Â »ç¶÷µéÀº protease / phosphatase¸¦ ÀÌ¿ëÇÏ¿© Phosphorylation / DephosphorylationÀ» ¹ß»ýÇÏ¿© ±× ÀÌÈÄ ±â´ÉÀ» È®ÀÎÇÕ´Ï´Ù. NEB¿¡¼­µµ ¾Æ·¡¿Í °°Àº protein PhosphatasesÀ» º¸À¯ÇÏ°í ÀÖ½À´Ï´Ù.
1. Lambda Protein Phosphatase (Lambda PP)
2. p-NitrophenyI Phosphate (PNPP)
3. Sodium Orthovanadate (Vanadate) .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes10

Authenticase¢â

±âÁ¸ Endonuclease I º¸´Ù ±â´ÉÀÌ ÇÑ ´Ü°è ´õ UPGRADE!

NEB»ç¿¡¼­ Á¦°øÇÏ´Â ½ÅÁ¦Ç° Authenticase
¤ý Authenticase´Â ÀÌÁß °¡´Ú DNAÀÇ 1-10 ¿°±â½Ö(bp) ¹üÀ§ÀÇ mismatch ¹× indel ºÎÀ§¸¦ ÀνÄÇÏ°í Àý´ÜÇÒ ¼ö ÀÖ´Â ±â´ÉÀ» °¡Áø nucleases È¿¼Ò ÀÔ´Ï´Ù.
¤ý C/C, T/C, A/C, T/G, G/G, T/T ¹× A/A¿Í °°Àº ´ÜÀÏ ¿°±â ºÎÀ§ mismatch ÀÎ½Ä (G/A Á¦¿Ü) .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ
NEBÀ̹ÌÁö DNAModifyingEnzymes11

Ligase

T4-Ligase ¸»°í DNA Substate Type¿¡ µû¸¥ NEBÀÇ Ligase Á¦Ç° »ç¿ëÇÏ°í Cloning È¿À²À» ³ô¿©º¸¼¼¿ä!

¤ý Blunt-end, single-base overhang¿¡ µû¸¥ ÃÖÀû¿¡ ligation Á¶°ÇÀ» ¸ÂÃá Blunt/TA Ligase Master Mix
¤ý (Cohesive-end[2-4bp])À» ºü¸¥ ½Ã°£¿¡ ligation ¹× transformation È¿À²À» Áõ°¡½Ãų ¼ö ÀÖ´Â Instant Sticky-end Ligase Master Mix .....Read more

º»»ç °¡±â NEBÁ¦Ç° ¹®ÀÇ