Colorimetric RapidLAMP 2X Master Mix with UDG

Molecular POCT

Colorimetric RapidLAMP 2X Master Mix with UDG

DNA ¶Ç´Â RNA(RT-LAMP) Ÿ°Ù µî¿Â ÁõÆø(LAMP; Loop-Mediated Isothermal Amplification)À» À§ÇÑ °£ÆíÇÑ ¿ø½ºÅÜ ¼Ö·ç¼Ç.

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  • ÁõÆø ½Ã º¸¶ó»ö¿¡¼­ ÆĶõ»öÀ¸·Î º¯ÇÏ´Â Ä÷¯¸¦ ºü¸£°í ¸íÈ®ÇÏ°Ô ½Ã°¢ÀûÀ¸·Î °¨Áö.

  • ¸¶½ºÅÍ ¹Í½º¿¡ ¿­¿¡ ¹Î°¨ÇÑ UDG¿Í dUTP°¡ Æ÷ÇÔµÇ¾î ±³Â÷¿À¿° À§Ç輺 °¨¼Ò.

  • °í³óµµÀÇ ÁõÆø ¾ïÁ¦Á¦¿¡¼­µµ ¶Ù¾î³­ ¼º´É.

  • ´Ù¾çÇÑ »ùÇà À¯Çü°ú ȣȯµÇ¸ç ´Ù¾çÇÑ °ËÃâ ¹æ¹ýÀ» Áö¿ø.

  • ±Ý¼Ó Áö½Ã¾àÀ» ÅëÇÑ Á¾Á¡(end-point) Ä÷¯ °¨Áö.

  • ¿­¿ø(heat source) ÀÌ¿Ü¿¡ ´Ù¸¥ µµ±¸°¡ ÇÊ¿äÇÏÁö ¾ÊÀ½.


Colorimetric RapidLAMP 2X Master Mix with UDG´Â LAMP ¹× RT-LAMP ¹ÝÀÀÀ» ºü¸£°í ½±°Ô °¨ÁöÇÒ ¼ö ÀÖµµ·Ï °¡½ÃÀûÀÎ ±Ý¼Ó Áö½Ã¾àÀÌ Æ÷ÇÔµÈ ÃÖÀûÈ­µÈ ¿ÏÃæ¿ë¾×¿¡ Bst 3.0 DNA ÁßÇÕÈ¿¼Ò¿Í WarmStart RTx ¿ªÀü»ç È¿¼Ò¸¦ ÃÖÀûÈ­ÇÑ Á¦Á¦ÀÔ´Ï´Ù. ¶ÇÇÑ ¸¶½ºÅÍ ¹Í½º¿¡ Æ÷ÇÔµÈ dUTP ¿Í UDG´Â ¹ÝÀÀ°£ ±³Â÷¿À¿°ÀÇ °¡´É¼ºÀ» °¨¼Ò½ÃÄÑ ÁÝ´Ï´Ù. ÀÌ ½Ã½ºÅÛÀº LAMP ¹ÝÀÀ Áß¿¡ ¸¶±×³×½·ÀÌ¿ÂÀÌ dNTPs¿¡¼­ ÆÄ»ýµÈ ÇÇ·ÎÀλ꿰(pyrophosphate)°ú ¹ÝÀÀÇÏ¿© ħÀü¹°À» »ý¼ºÇÏ´Â °ÍÀ» ±â¹ÝÀ¸·Î DNA ÁõÆøÀ» ºü¸£°í ¸íÈ®ÇÏ°Ô ½Ã°¢ÀûÀ¸·Î °¨ÁöÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÇ¾ú½À´Ï´Ù. ¿ë¾× ³» ¸¶±×³×½· ÀÌ¿ÂÀÇ °¨¼Ò·Î ÀÎÇØ º¸¶ó»ö¿¡¼­ ÆĶõ»öÀ¸·ÎÀÇ »ö»óÀÇ º¯È­°¡ ÀϾ´Ï´Ù. ÀÌ ¸¶½ºÅÍ ¹Í½º´Â ¸ðµç LAMP ¶Ç´Â RT-LAMP ¹ÝÀÀ¿¡ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, °¡¿­µÈ è¹ö¿Í ½Ã·á¸¸À» ÇÊ¿ä·Î Çϸç, 30-40ºÐ ³»¿¡ ´«À¸·Î ¾ç¼º ÁõÆøÀ» ÆÇ´Ü ÇÒ ¼ö ÀÖ½À´Ï´Ù.

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Á¦Ç°¹øÈ£ Á¦Ç°¸í ³óµµ Size
RL1000--01 Colorimetric RapidLAMP 2X Master Mix with UDG 2X 1¡¿1.05ml (100 reactions)
RL1000--02 2¡¿1.05ml (200 reactions)
RL1000--05 5¡¿1.05ml (500 reactions)

Colorimetric RapidLAMP 2X Master Mix with UDG

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

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Negative reactions are indicated in purple and positive reactions are indicated by change to blue.

Features

  • Fast, clear, purple-to-blue visible detection of amplification.

  • Reduce risk of carryover contamination with thermolabile UDG and dUTP included.
    • Robust performance in high concentrations of amplification inhibitors.

  • Compatible with different sample types and supports multiple detection methods.

  • End-point colorimetric detection via a metal indicator.

  • No other instrument is required for this assay, except for a heat source.


Colorimetric RapidLAMP 2X Master Mix with UDG is an optimized formulation of Bst 3.0 DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer reaction solution containing a visible metal indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions. This system is designed to provided a fast, clear visible detection of DNA amplification based on the magnesium ions react with pyrophosphate derived from dNTPs during LAMP reaction and generate a precipitate. The resulting depletion of magnesium ions in solution results in a color change from purple to blue. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, and positive amplification judged by eye in 30–40 minutes.

Ordering information

Cat no. Product Name concentration Size
RL1000--01 Colorimetric RapidLAMP 2X Master Mix with UDG 2X 1¡¿1.05ml (100 reactions)
RL1000--02 2¡¿1.05ml (200 reactions)
RL1000--05 5¡¿1.05ml (500 reactions)