3D Cell Culture incubator : CLINOSTAR™
ClinoStar, Stress-free 3-D Cell culture system은 Clinostat 방식으로, 회전을 사용하여 중력의 영향을 무효화 시켜 스트레스가 없는 환경에서 세포를 배양하는 장비입니다. ClinoStar 는 조직과 유사한 구조와 생체 내 기능을 통해 3D로 세포를 성장시킵니다. 회전으로 생기는 기체 및 영양소의 활발한 교환은 재현성이 높은 오가노이드 및 스페로이드를 생성을 보장합니다. 6개의 clinostat을 장착해 각각은 직관적 인 소프트웨어를 사용하여 제어되며, 실시간으로 세포의 성장을 관찰할 수 있습니다.
ClinoReactor is a bioreactor with a fixed 10 mL culture chamber, that is supplied sterile in a sealed and wrapped package for convenient use and to limit the infection risk. The humidification beads supply water for the culture chamber, which eliminate the need for water in the incubator. To prevent cell, protein and drug absorption, the bioreactor is made from low bind polypropylene and polystyrene. The optically clear properties of the polystyrene enables microscopy of the spheroids or organoids in a closed environment.
Push to open
For convenient hands free opening and reduced contamination risk.
Can contain and maintain over 350 mature cell Constructs.
Fits anywhere, even in your laf-bench.
The large heating element and fan ensure an equal distribution and low bariability of heat and CO across the chamber.
An ethernet connection allows for direct internet access or receive software updates via the control unit.
Automatic UBC - decontamination cycles.
How to work with CLINOSTAR
Spheriod and organoid development
1. Select aggregation method
Following 2D cell expansion, the cells can be seeded in the ClinoReactor with one of three approaches: single cell suspension or initial aggregates preformed in hydrogel or micropattern plates.
2. Transfer to ClinoReactor
In the ClinoStar® system, you can use your regular cell culture media and supplements, obviate the need for specialised media, scaffolds, ECM substitutes or growth factors.
3. Cultivate in ClinoStar®
In the cultivation phase, cell culture media is routinely renewed, and irregular constructs are removed from the ClinoReactor to ensure uniform growth. Check that your constructs are functional against a parameter that can be assayed in vivo.
4. Mature the constructs
When the cell lines have recovered their functionality, they are considered mature and ready for experiments. For HEPG2/C3A cells each ClinoReactor will hold around 350 spheroids after 18 days of maturation.
|Close mechanism||Push - click - swing open|
|Open mechanism||Push to close - click|
|Speed range(rpm)||0 - 100|
|Direction||Clockwise or anti clockwise|
|Temperature range||From 6 to 20℃ above ambient|
|Temperature accuracy||± 0.25℃|
|Co2 range[Vol.-% co2]||0 - 10%|
|Co2 calibration||Factory calibrated for 10 years|
|Cameras||6(placed opposite to each axle)|
|Camera resolution||5 Megapixel|
|Lighting||Front and back LEDs for each axle|
|Incorporated method||UV-C LED 300mA|
|Time||User activated (2hours runtime)|
|Screen resolution||1920 x 1200|
|Units to control||50|
|Paused while door is open||UV-C emitting LED, fan and CO₂|
|USB||Back and front (in door)|
|CO₂||Ethernet port provide 1500V insulation|
|Space saving configuration||Up to 3 units can be stacked on top of each other|
(the stacking bar must be used to increase their stability)
|Rated Voltage [V]||100 - 240 V|
|Power frequency [Hz]||50 - 60 Hz|
|Nominal power [A]||1.8 A|
|Power cord length||2 meters|
|Appliance Class||Class Ⅰ equipment|
|30002||ClinoStar||-||Celvivo||The clinoStar enables you to culture cells in 3D. Intuiative software controls the temperature,CO2 level and each of the 6 clinostatmotors independently.k Cameras allow you to see your experiments without opening the dor.|
|31001||Control Unit||-||Celvivo||-Additional control unit; tablet with operating software pre-installed|
|10004-12||ClinoReactor 10ml - 12 Pack||-||Celvivo||-12x ClinoReactors with 10ml incubation chamber and solid waterbeads|