3D Cell Culture incubator : CLINOSTAR¢â
ClinoStar, Stress-free 3-D Cell culture systemÀº Clinostat ¹æ½ÄÀ¸·Î, ȸÀüÀ» »ç¿ëÇÏ¿© Áß·ÂÀÇ ¿µÇâÀ» ¹«È¿È ½ÃÄÑ ½ºÆ®·¹½º°¡ ¾ø´Â ȯ°æ¿¡¼ ¼¼Æ÷¸¦ ¹è¾çÇÏ´Â ÀåºñÀÔ´Ï´Ù. ClinoStar ´Â Á¶Á÷°ú À¯»çÇÑ ±¸Á¶¿Í »ýü ³» ±â´ÉÀ» ÅëÇØ 3D·Î ¼¼Æ÷¸¦ ¼ºÀå½Ãŵ´Ï´Ù. ȸÀüÀ¸·Î »ý±â´Â ±âü ¹× ¿µ¾ç¼ÒÀÇ È°¹ßÇÑ ±³È¯Àº ÀçÇö¼ºÀÌ ³ôÀº ¿À°¡³ëÀÌµå ¹× ½ºÆä·ÎÀ̵带 »ý¼ºÀ» º¸ÀåÇÕ´Ï´Ù. 6°³ÀÇ clinostatÀ» ÀåÂøÇØ °¢°¢Àº Á÷°üÀû ÀÎ ¼ÒÇÁÆ®¿þ¾î¸¦ »ç¿ëÇÏ¿© Á¦¾îµÇ¸ç, ½Ç½Ã°£À¸·Î ¼¼Æ÷ÀÇ ¼ºÀåÀ» °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.
Features
ClinoReactor is a bioreactor with a fixed 10 mL culture chamber, that is supplied sterile in a sealed and wrapped package for convenient use and to limit the infection risk. The humidification beads supply water for the culture chamber, which eliminate the need for water in the incubator. To prevent cell, protein and drug absorption, the bioreactor is made from low bind polypropylene and polystyrene. The optically clear properties of the polystyrene enables microscopy of the spheroids or organoids in a closed environment.
- 1
Push to open
For convenient hands free opening and reduced contamination risk.
- 2
Adjustable light
Can contain and maintain over 350 mature cell Constructs.
- 3
Small footprint
Fits anywhere, even in your laf-bench.
- 4
Uniform environment
The large heating element and fan ensure an equal distribution and low bariability of heat and CO across the chamber.
- 5
Connectivity options
An ethernet connection allows for direct internet access or receive software updates via the control unit.
- 6
Decontamination
Automatic UBC - decontamination cycles.
How to work with CLINOSTAR
Spheriod and organoid development
1. Select aggregation method
Following 2D cell expansion, the cells can be seeded in the ClinoReactor with one of three approaches: single cell suspension or initial aggregates preformed in hydrogel or micropattern plates.
2. Transfer to ClinoReactor
In the ClinoStar¢ç system, you can use your regular cell culture media and supplements, obviate the need for specialised media, scaffolds, ECM substitutes or growth factors.
3. Cultivate in ClinoStar¢ç
In the cultivation phase, cell culture media is routinely renewed, and irregular constructs are removed from the ClinoReactor to ensure uniform growth. Check that your constructs are functional against a parameter that can be assayed in vivo.
4. Mature the constructs
When the cell lines have recovered their functionality, they are considered mature and ready for experiments. For HEPG2/C3A cells each ClinoReactor will hold around 350 spheroids after 18 days of maturation.
Specification
Clinostar
Door | |
Close mechanism | Push - click - swing open |
Open mechanism | Push to close - click |
Axles | |
Capacity | 6 axles |
Speed range(rpm) | 0 - 100 |
Speed Accuracy | ¡¾1% |
Direction | Clockwise or anti clockwise |
Control | Independent |
Temperature data | |
Temperature range | From 6 to 20¡É above ambient |
Temperature accuracy | ¡¾ 0.25¡É |
Co2-data | |
Co2 range[Vol.-% co2] | 0 - 10% |
Co2 measurement | IR |
Co2 calibration | Factory calibrated for 10 years |
Monitoring | |
Cameras | 6(placed opposite to each axle) |
Camera resolution | 5 Megapixel |
Lighting | Front and back LEDs for each axle |
Decontamination | |
Incorporated method | UV-C LED 300mA |
Time | User activated (2hours runtime) |
Controller | |
Device | Tablet |
Communication method | Wi-Fi,Ethernet |
Screen size | 10,1" |
Screen resolution | 1920 x 1200 |
Units to control | 50 |
Safety | |
Paused while door is open | UV-C emitting LED, fan and CO©ü |
Connectors | |
USB | Back and front (in door) |
Network | Ethernet (RJ-45) |
CO©ü | Ethernet port provide 1500V insulation |
Footprint | |
Space saving configuration | Up to 3 units can be stacked on top of each other (the stacking bar must be used to increase their stability) |
Rated Voltage [V] | 100 - 240 V |
Power frequency [Hz] | 50 - 60 Hz |
Nominal power [A] | 1.8 A |
Power cord length | 2 meters |
Appliance Class | Class ¥° equipment |
Pollution Degree | 2 |
Overvoltage category | ¥± |
Product information
Cat No. | Ç°¸í | ±Ô°Ý | Á¦Á¶»ç | Á¦Ç°Á¤º¸ |
---|---|---|---|---|
30002 | ClinoStar | - | Celvivo | The clinoStar enables you to culture cells in 3D. Intuiative software controls the temperature,CO2 level and each of the 6 clinostatmotors independently.k Cameras allow you to see your experiments without opening the dor. |
31001 | Control Unit | - | Celvivo | -Additional control unit; tablet with operating software pre-installed |
10004-12 | ClinoReactor 10ml - 12 Pack | - | Celvivo | -12x ClinoReactors with 10ml incubation chamber and solid waterbeads |