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About Us¾î¹Ù¿ô ¾î½º
Synthego»çÀÇ Genome Engineering PlatformÀº À¯ÀüÀÚ ÆíÁý ±â¼ú ¹× ¼¼Æ÷ ±â¹Ý ¸ðµ¨ ¿¬±¸¸¦ ÃËÁøÇϱâ À§ÇØ ÃÖ°íÀÇ ±Ô¸ð¿Í Ç°ÁúÀ» Á¦°øÇÕ´Ï´Ù. Synthego»ç¿¡¼´Â À¯ÀüÀÚ ±³Á¤ ¼ºñ½º ¹× °ü·Ã Á¦Ç° Á¦°ø »Ó¸¸ ¾Æ´Ï¶ó, NGS±ÞÀÇ ºÐ¼® ¼ºñ½ºÀÎ ICE analysis ½Ã½ºÅÛ°ú, ÀÚüÀûÀÎ sgRNA Design toolÀ» Á¦°øÇÏ°í ÀÖÀ¸¸ç, Genome Editing ±â¹ý°ú °ü·ÃµÈ ´Ù¾çÇÑ resource¸¦ Á¦°øÇÏ°í ÀÖ½À´Ï´Ù.
Synthetic sgRNA | ÇÕ¼ºÇÏ°íÀÚ ÇÏ´Â sgRNAÀÇ ¿°±â¼¿ Á¤º¸¸¦ Àü´ÞÇØ Áֽøé 1.5 nmol, 3 nmol, 10 nmol, 20 nmol, 50 nmol, 100 nmol scale·Î ÇÕ¼ºÇÏ´Â ¼ºñ½ºÀÔ´Ï´Ù. |
Advanced RNA | ¨ç Cas Variant »ç¿ë, ¨è Long length target sequence ÇÊ¿ä, ¨é Çü±¤ ¶Ç´Â ´Ù¸¥ º¯Çüü¸¦ guide RNA¿¡ °áÇÕÇÏ´Â °æ¿ì µî ƯÀÌÀû sgRNA¸¦ ÇÕ¼ºÇÏ´Â ¼ºñ½ºÀÔ´Ï´Ù. |
Gene Knockout Kit | Ÿ°Ù À¯ÀüÀÚ¿¡ ´ëÇÑ Editing efficiency Çâ»óÀ» À§ÇؽÃÅ°±â À§ÇØ Multiple sgRNA¸¦ Á¦°øÇÏ´Â kit·Î, À¯ÀüÀÚ Á¤º¸¸¦ Á¦°øÇØ Áֽøé ÇÕ¼º sgRNA ¹× ½ÇÇè¿¡ ÇÊ¿äÇÑ ±âº»ÀûÀÎ ½Ã¾àµéÀ» Á¦°øÇÏ´Â ¼ºñ½ºÀÔ´Ï´Ù. |
sgRNAÀÇ ¾ÈÁ¤¼º°ú Editing È¿À²À» Çâ»ó½ÃÅ°±â À§ÇÑ modified guide RNA* ÇÕ¼º °¡´ÉÇÕ´Ï´Ù.
* Modification : 2'-O-methyl 3' phosphorothioate modifications in the first and last 3 nucleotides (end-modification)
CRISPR À¯ÀüÀÚº¯Çü ½ÇÇèÀº ¼ö¸¹Àº º¯¼ö¿Í ¸¹Àº ÇüÁúÀüȯ ÃÖÀûÈ Á¶°ÇÀÌ ÀÖ¾î ÃʽÉÀÚ°¡ ½ÇÇèÀ» ¹è¿ì´Âµ¥ 6°³¿ù ÀÌ»ó ¼Ò¿äµÇ¸é ¼º°øÇϱâ±îÁö ¾à 8¹øÀÇ ½ÇÆи¦ ÇÏ´Â ³À̵µ°¡ ³ôÀº ½ÇÇèÀÔ´Ï´Ù. Synthego»ç´Â CRISPR½ÇÇèÀÇ Àü¹®°¡Áý´ÜÀ¸·Î¼ ÀÌ¹Ì ¼ö¸¹Àº º¯¼ö°¡ ÃÖÀûȵǾî ÀÖ¾î ´Ü±â°£¿¡ CRISPRÀ¯ÀüÀÚº¯Çü¼¼Æ÷ÁÖ Á¦ÀÛÀÌ °¡´ÉÇÕ´Ï´Ù. CRISPR À¯ÀüÀÚ º¯Çü ½ÇÇèÀº Synthego»ç¿¡ ¸Ã±â½Ã°í Á» ´õ °úÇÐÀû ¹ß°ß¿¡ ÁýÁßÇغ¸¼¼¿ä.
1) Knockout & Knock-In Cell Pool
- Edited and Unedited mixed cell·Î ±¸¼º
- Genome editing efficiency °¡ Æò±Õ 70~80% ÀÌ»óÀ¸·Î °£´ÜÇÑ ºÐ¼®¿¡ ¹Ù·Î È°¿ë °¡´É
1) Knockout & Knock-In Cell Clone
- 100% edited cell ·Î ±¸¼ºµÈ Cell CloneÀ¸·Î ±¸¼º
- ÃÖÀûÀÇ limiting dilution ¹æ¹ýÀ» È®¸³ÇÏ¿©, Á»´õ ¸íÈ®ÇÑ »óÈ£°ü°è ÆÄ¾Ç ¿¬±¸ ¹× ºÐ¼®¿¡ È°¿ë
1) Immortalized Cell
- ´Ù¾çÇÑ Cell Line (Á¦ÀÛ ¹®Àǽà ±âÀç)
2) iPSC
- PGP1 (male line) or 802-30F (female line) stem cell line
´Ù¾çÇÑ À¯ÀüÀÚÀÇ Knockout ¾ç»óÀ» º¸°íÀÚ ÇϽô ¿¬±¸ÀÚ
- 20 °³ ÀÌ»ó À¯ÀüÀÚÀÇ Knockout CellÀ» °¢°¢ Á¦°ø
- °³º° Cell pool Á¦ÀÛ ºñ¿ëº¸´Ù ¸Å¿ì Àú·ÅÇÑ °¡°Ý
Workflow
sgRNA | ¹®ÀÇ | Target Sequence Modification Yield | ¹ßÁÖ [Yield¿¡ µû¶ó 10ÀÏ~20ÀÏ] | °í°´¿¡°Ô Àü´Þ |
Engineered Cell | Target Gene Editing Type (KO or KI) Cell Line | ¹ßÁÖ [Cell Pool : ~5ÁÖ] [Cell Clone: ~12ÁÖ] KIÀÇ °æ¿ì Ãß°¡ ±â°£ÀÌ ¼Ò¿äµÉ ¼öµµ ÀÖ½À´Ï´Ù. |