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DNA ¶Ç´Â RNA(RT-LAMP) Ÿ°Ù µî¿Â ÁõÆø(LAMP; Loop-Mediated Isothermal Amplification)À» À§ÇÑ °£ÆíÇÑ ¿ø½ºÅÜ ¼Ö·ç¼Ç.
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¸¶½ºÅÍ ¹Í½º¿¡ ¿¿¡ ¹Î°¨ÇÑ UDG¿Í dUTP°¡ Æ÷ÇÔµÇ¾î ±³Â÷¿À¿° À§Ç輺 °¨¼Ò.
°í³óµµÀÇ ÁõÆø ¾ïÁ¦Á¦¿¡¼µµ ¶Ù¾î³ ¼º´É.
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±Ý¼Ó Áö½Ã¾àÀ» ÅëÇÑ Á¾Á¡(end-point) Ä÷¯ °¨Áö.
¿¿ø(heat source) ÀÌ¿Ü¿¡ ´Ù¸¥ µµ±¸°¡ ÇÊ¿äÇÏÁö ¾ÊÀ½.
Colorimetric RapidLAMP 2X Master Mix with UDG´Â LAMP ¹× RT-LAMP ¹ÝÀÀÀ» ºü¸£°í ½±°Ô °¨ÁöÇÒ ¼ö ÀÖµµ·Ï °¡½ÃÀûÀÎ ±Ý¼Ó Áö½Ã¾àÀÌ Æ÷ÇÔµÈ ÃÖÀûÈµÈ ¿ÏÃæ¿ë¾×¿¡ Bst 3.0 DNA ÁßÇÕÈ¿¼Ò¿Í WarmStart RTx ¿ªÀü»ç È¿¼Ò¸¦ ÃÖÀûÈÇÑ Á¦Á¦ÀÔ´Ï´Ù. ¶ÇÇÑ ¸¶½ºÅÍ ¹Í½º¿¡ Æ÷ÇÔµÈ dUTP ¿Í UDG´Â ¹ÝÀÀ°£ ±³Â÷¿À¿°ÀÇ °¡´É¼ºÀ» °¨¼Ò½ÃÄÑ ÁÝ´Ï´Ù. ÀÌ ½Ã½ºÅÛÀº LAMP ¹ÝÀÀ Áß¿¡ ¸¶±×³×½·ÀÌ¿ÂÀÌ dNTPs¿¡¼ ÆÄ»ýµÈ ÇÇ·ÎÀλ꿰(pyrophosphate)°ú ¹ÝÀÀÇÏ¿© ħÀü¹°À» »ý¼ºÇÏ´Â °ÍÀ» ±â¹ÝÀ¸·Î DNA ÁõÆøÀ» ºü¸£°í ¸íÈ®ÇÏ°Ô ½Ã°¢ÀûÀ¸·Î °¨ÁöÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÇ¾ú½À´Ï´Ù. ¿ë¾× ³» ¸¶±×³×½· ÀÌ¿ÂÀÇ °¨¼Ò·Î ÀÎÇØ º¸¶ó»ö¿¡¼ ÆĶõ»öÀ¸·ÎÀÇ »ö»óÀÇ º¯È°¡ ÀϾ´Ï´Ù. ÀÌ ¸¶½ºÅÍ ¹Í½º´Â ¸ðµç LAMP ¶Ç´Â RT-LAMP ¹ÝÀÀ¿¡ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, °¡¿µÈ è¹ö¿Í ½Ã·á¸¸À» ÇÊ¿ä·Î Çϸç, 30-40ºÐ ³»¿¡ ´«À¸·Î ¾ç¼º ÁõÆøÀ» ÆÇ´Ü ÇÒ ¼ö ÀÖ½À´Ï´Ù.
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Á¦Ç°¹øÈ£ | Á¦Ç°¸í | ³óµµ | Size |
---|---|---|---|
RL1000--01 | Colorimetric RapidLAMP 2X Master Mix with UDG | 2X | 1¡¿1.05ml (100 reactions) |
RL1000--02 | 2¡¿1.05ml (200 reactions) | ||
RL1000--05 | 5¡¿1.05ml (500 reactions) |
Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
Negative reactions are indicated in purple and positive reactions are indicated by change to blue.
Features
Fast, clear, purple-to-blue visible detection of amplification.
Reduce risk of carryover contamination with thermolabile UDG and dUTP included. • Robust performance in high concentrations of amplification inhibitors.
Compatible with different sample types and supports multiple detection methods.
End-point colorimetric detection via a metal indicator.
No other instrument is required for this assay, except for a heat source.
Colorimetric RapidLAMP 2X Master Mix with UDG is an optimized formulation of Bst 3.0 DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer reaction solution containing a visible metal indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions. This system is designed to provided a fast, clear visible detection of DNA amplification based on the magnesium ions react with pyrophosphate derived from dNTPs during LAMP reaction and generate a precipitate. The resulting depletion of magnesium ions in solution results in a color change from purple to blue. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, and positive amplification judged by eye in 30–40 minutes.
Ordering information
Cat no. | Product Name | concentration | Size |
---|---|---|---|
RL1000--01 | Colorimetric RapidLAMP 2X Master Mix with UDG | 2X | 1¡¿1.05ml (100 reactions) |
RL1000--02 | 2¡¿1.05ml (200 reactions) | ||
RL1000--05 | 5¡¿1.05ml (500 reactions) |