NEBNext® Immune Sequencing Kit (Human)
Feature
With the NEBNext® Immune Sequencing Kit (Human), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy. Characterize BCR light, BCR heavy, TCRα and TCRβ chains. This kit includes UMIs for source-molecule identification.
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Unlock the immune system’s complexity with a deeper analysis of receptor sequences
- 2
Enrich for and sequence both B cell receptors (BCR) and T cell receptors (TCR)
- 3
Generate full-length immune gene repertoires of B and T cells
- 4
Accurately quantify transcripts with unique molecular identifiers (UMIs)
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Analyze data using a bioinformatic workflow based on the open-source pRESTO toolkit (tutorial)
