Feature

The NEBNext® Library Quant Kit for Illumina® has been updated to include six standards, enabling a broader standard curve. For users who are familiar with the previous four standards, numbering has changed (e.g., what was once Standards 1-4 is now Standards 2-5). Both the four-standard and six-standard protocols are supported; see the Manual for details.

Accurate quantitation of next-generation sequencing (NGS) libraries is essential for maximizing data output and quality from each sequencing run. For Illumina® sequencing specifically, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. Further, amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced. The NEBNext® Library Quant Kit has been optimized to provide substantial performance and workflow improvements to qPCR-based library quantitation.

The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.
·   Compatible with a broad range of input amounts: 10 ng - 1µg
·   Suitable for low-quality or high-quality RNA
·   Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available with RNAClean® beads.