NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Feature
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. Strand-specific/directional methods for sequencing RNA provide information on the DNA strand from which the RNA strand was transcribed. This is useful for many reasons including: Identification of antisense transcripts, determination of the transcribed strand of noncoding RNA, and measurement of ________expression________ levels of coding or noncoding overlapping transcripts. Overall, the ability to determine the originating strand can substantially enhance the value of a RNA-seq experiment. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application.
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Get more of what you need, with the highest library yields
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Generate high quality libraries even when you have only limited amounts of input RNA:
· 10 ng – 1 µg Total RNA (polyA mRNA workflow)
· 5 ng – 1 µg Total RNA (rRNA depletion workflow) - 3
Minimize bias, with fewer PCR cycles required
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Increase the complexity and transcript coverage of your libraries
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Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
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Rely on robust performance, even with low quality RNA, including FFPE
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Compatible with NEBNext poly(A) mRNA Isolation, rRNA Depletion reagents and multiplexing adaptors and primers.
