NEBNext¢ç Enzymatic Methyl-seq Kit
Feature
The NEBNext Enzymatic Methyl-seq Kit provides a high-performance enzyme-based alternative to bisulfite conversion for methylome analysis using Illumina¢ç sequencing. Libraries are prepared using as little as 10 ng input DNA and the supplied NEBNext Ultra II reagents and the optimized EM-seq Adaptor. TET2 then oxidizes 5-mC and 5-hmC, providing protection from deamination by APOBEC in the next step. In contrast, unmodified cytosines are deaminated to uracils. Libraries are then amplified using a NEBNext master mix formulation of Q5U¢ç (a modified version of Q5¢ç High-Fidelity DNA Polymerase), and sequenced using Illumina instrumentation. The consistently high conversion performance and minimized DNA damage with the EM-seq protocol, in combination with highly efficient Ultra II library prep, result in superior detection of CpGs with fewer sequencing reads.
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Superior sensitivity of detection of 5-mC and 5-hmC
- 2
Greater mapping efficiency
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More uniform GC coverage
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Detection of more CpGs with fewer sequence reads
- 5
Uniform dinucleotide distribution
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High-efficiency library preparation, with larger library insert sizes
- 7
Conversion module also available separately
Product Information
NEBNext¢ç Enzymatic Methyl-seq Kit
Cat # | Size |
---|---|
E7120S | 24 reactions |
E7120L | 96 reactions |