NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina
Feature
The NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5® Master Mix for uniform, high-fidelity library amplification. Please note that adaptors and primers are not included in the kit and are available separately.
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Fragmentation, end repair and dA-tailing reagents in a single enzyme mix
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A single fragmentation protocol, regardless of DNA input amount or GC content
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Input amounts: 100 pg–500 ng
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Input DNA can be in water, Tris or TE
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Workflow: ~ 2.5 hours, with < 15 minutes hands-on time
