DNA Gel Extraction & Cleanup kit
Feature
DNA Gel Extraction
The Monarch DNA Gel Extraction Kit reliably purifies up to 5 µg of concentrated, high quality DNA from agarose gels. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design eliminates buffer retention and carryover of contaminants, enabling elution in volumes as low as 6 µl and with fewer steps. Monarch Buffers have been optimized, and do not require pH monitoring. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving a step. Eluted DNA is ready for use in restriction digestion, DNA sequencing, PCR, ligation, and other enzymatic manipulations.
DNA Cleanup
The Monarch PCR & DNA Cleanup Kit reliably purifies up to 5 µg of concentrated, high quality DNA from PCR and other enzymatic reactions. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns provided with each kit ensure zero buffer retention and no carryover of contaminants, enabling elution in volumes as low as 6 µl. Monarch Buffers have been optimized, and do not require pH monitoring. Eluted DNA is ready for use in restriction digests, DNA sequencing, PCR, ligation, and other enzymatic manipulations.
Specification
Monarch PCR & DNA Cleanup Kit
Binding Capacity: | up to 5 ¥ìg |
DNA Size Range: | ~50 bp to 25 kb DNA ¡Ã 15 bp to 25 kb (dsDNA) and DNA ¡Ã 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol. |
Typical Recovery: | DNA (50 bp to 10 kb): 70–90% DNA (11–25 kb): 50–70% ssDNA ¡Ã 18 nt and dsDNA ¡Ã 15 bp: 70-85% |
Elution Volume: | ¡Ã 6 ¥ìl |
Purity: | A260/280 ¡Ã 1.8 |
Protocol Time: | 5 min of spin and incubation time |
Common Downstream Applications: | ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |
Monarch DNA Gel Extraction Kit
Binding Capacity: | up to 5 ¥ìg |
DNA Size Range: | ~50 bp to 25 kb |
Typical Recovery: | DNA (50 bp to 10 kb): 70–90% DNA (11–25 kb): 50–70% |
Elution Volume: | ¡Ã 6 ¥ìl |
Purity: | A260/280 ¡Ã 1.8 |
Protocol Time: | 10 min of spin and incubation time. |
Common Downstream Applications: | ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |
Product Information
DNA Gel Extraction & Cleanup kit
Monarch¢ç DNA Elution Buffer | Cat No. | SIze |
---|---|---|
T1016L | 25 ml | |
Monarch¢ç DNA Gel Extraction Kit | Cat No. | SIze |
T1020S | 50 preps | |
T1020L | 250 preps | |
Monarch¢ç DNA Cleanup Columns (5 ¥ìg) | Cat No. | SIze |
T1034L | 100 columns | |
Monarch¢ç DNA Wash Buffer | Cat No. | SIze |
T1032L | 25 ml |