Moxi Z
Mini Automated Cell Counter Kit
![[ORFLO]Moxi Z](https://philekorea1.cafe24.com/bizdemo76095/img/_images/list_img/mp14/sub2/list2/1.jpg)
Features
- 1
Improve downstream results with better quality control.
Moxi Z is the only automated cell counter that combines the Coulter Principle typically used in high-end cell counters with a patented thin-film sensor technology to allow for highly accurate (> 95%) and repeatable particle counting and sizing for a broad range of cell types – from mammalian cells to cells as small as wine yeast and more. Since today's workflows demand accurate quality control of samples, determining cell counts precisely has a significant impact on outcomes and downstream costs.
- 2
Produce repeatable cell counts with >95% accuracy in just 8 seconds.
This ultra-small instrument uses patented microfluidic thin-film cassettes that enable automatic load and measure operation. The resulting single cell volumetric measurements are completely technique-independent. It provides the ideal alternative to the tedious manual counting associated with cytometers, or the inaccurate results associated with image-based automated cell counters (typical accuracies of 75-80%).
- 3
Assess cell culture health without reagents or dyes.
Moxi Z also automatically reports a unique cell health assessment index – MPI (Moxi Population Index) – without the need for reagents or dyes. MPI is a ratio of the cell population of interest relative to the entire particle distribution in that sample, factoring in dead cells that have shrunken or broken apart as well as other debris and contaminants in the sample. For monodisperse mammalian cultures this is closely correlated to the overall health of the sample.In the case of mixed cell populations, it's provides an assessment of the relative fraction of the largest sized population in the sample. Unlike staining-based viability methods that focus on the uptake by dead cells and do not account for contaminants and other debris, MPI provides insight on the primary population of interest (based on size) and its relationship to ALL other particles in the sample.
- 4
Measure the smallest cells at very high concentrations.
Using the new Type S cassette, you can now accurately measure the smallest cells of any automated cell counter (down to 3um average diameter) at concentrations of up to 2.5e+6/ml. This means the new Type S cassette can measure Yeast (including wine yeast) and non-spherical particles down to 14 fL in volume.
Results in just 8 seconds
Ultra-small and ultra-easy to use (no manual counting, no focusing of any kind)
Complete, high-resolution cell size/count histogram
Handles "de-aggregation" and coincidence events
Post-processing/analysis of count distributions with user-adjustable regions/gates
Assess cell health (MPI) without the need for reagents like Trypan blue
How It Works
The operating principle behind the Moxi GO II Flow Cytometers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 488nm (MXG102) solid state diode laser with and with the following emission filters - 525/45nm (e.g. FITC, GFP, Alexa 488) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
Step 1:
Insert cassette into Moxi Z.
![[ORFLO]Moxi Z](https://philekorea1.cafe24.com/bizdemo76095/img/_images/list_img/mp14/sub2/list2/1_1.jpg)
Step 2:
Pipette 75 μL sample into cassette. Touch Screen.
![[ORFLO]Moxi Z](https://philekorea1.cafe24.com/bizdemo76095/img/_images/list_img/mp14/sub2/list2/1_2.jpg)
Finished! Automatic, hands-free measurement in just 8 seconds.
Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into MoxiZ, and load Sample 2.
Data:
For each measurement, a complete histogram is displayed as well as cell count, average cell size and Moxi Population Index. The raw waveform from the most recent cell count, containing each cell spike, is also stored on the Moxi Z and can be both viewed on the Moxi Z and uploaded to the PC for additional analysis. The Moxi Z also performs sophisticated curve fitting analysis to more accurately determine cell counts in the event of overlapping populations of cells (or debris), significantly improving the accuracy of the volumetric counts produced by the Moxi Z. Data for 500 samples may be stored on the unit. All data can be transferred to a PC/Mac via Bluetooth. No special software is required for the PC since date files are Excel®-compatible. Sophisticated PC/Mac compatible analys software is included with each Moxi Z. Cell health may be assessed by comparing differences in histograms from culture to culture.
