qPCR Cycler
:MIC Magnetic Induction Cycler
![MIC Magnetic Induction Cycler](http://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/1.webp)
MIC´Â ¼¼°è ÃÖÃÊ Magnetic Induction ¹æ½ÄÀÇ Real-time PCR ÀÔ´Ï´Ù. ºü¸£°í Á¤È®ÇÑ qPCR °á°ú¸¦ º¸ÀåÇÕ´Ï´Ù.
2kgÀÇ ÀÛÀº Å¥ºê ¾È¿¡ ´ã°ÜÁø °·ÂÇÑ ±â¼úÀ» °æÇèÇØ º¸¼¼¿ä.
Feature
- 1
¡¤ Magnetic Induction Technology¸¦ ÀÌ¿ëÇÑ ¿È¿À² ±Ø´ëÈ
¡¤ Channel º° µ¶¸³µÈ high intensity LED ¹× photodetector
¡¤ °íÁ¤µÈ optic module·Î calibration ¹× align ºÒÇÊ¿ä
¡¤ Rotor ¹æ½ÄÀÇ sample ȸÀüÀ¸·Î acquisition time 1ÃÊ ¼Ò¿ä
¡¤ ¿¹¿ ½Ã°£ÀÌ ÇÊ¿ä¾ø¾î ³¶ºñÇÏ´Â ½Ã°£ ¾øÀ½ - 2
¡¤ ȸÀüÇüÀÇ ¾Ë·ç¹Ì´½ ·ÎÅÍ·Î Á¤Àû / µ¿Àû ¿Âµµ±ÕÀϼº Á¦°ø
¡¤ ¿Âµµ Á¤È®¼º accuracy of ¡¾ 0.25 ¨¬C
¡¤ Well °£ ¿Âµµ ±ÕÀϼº uniformity of
¡¾ 0.05 ¨¬C maximum (MIC-4+HRM) ¡¾ 0.1 ¨¬C maximum (MIC-2 and MIC-4)
¡¤ HRMÀ» ÀÌ¿ëÇÑ Class IV SNP genotypingÀ» ½±°Ô ºÐ·ù - 3
¡¤ Bluetooth ¶Ç´Â USB ´ÙÁß¿¬°á °¡´É (ÃÖ´ë 10´ë)
¡¤ 48 - 96 - 192 - 480 ±îÁö È®À强 ¿ëÀÌ
¡¤ °³º° µ¥ÀÌÅÍÀÇ ÅëÇÕ ºÐ¼® °¡´É (ÃÖ´ë 480 samples)
¡¤ ¿ÀÇÂÇü ¼ÒÇÁÆ®¿þ¾î (Freeware) - 4
¡¤ ¼Õ¾È¿¡ µé¾î¿À´Â ÀÛÀº qPCR machine
¡¤ °¡·Î x ¼¼·Î x ³ôÀÌ / ¹«°Ô : 15 cm x 15 cm x 13 cm / 2 kg
¡¤ °íÁ¤µÈ optic module·Î calibration ¹× align ºÒÇÊ¿ä
¡¤ 0.1 §¢ qPCR Æ©ºê¸¦ ÀÌ¿ëÇÏ¿© total volume 5~30 §¡ °¡´É
¡¤ 4-strip tubeÀÇ °³º° rack Æ÷ÀåÀ¸·Î multi pipette ºÐÁÖ°¡´É
Application
Absolute Quantification
Standard curve¸¦ »ç¿ëÇϸé, À¯ÀüÀÚÀÇ Àý´ë·®À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. ¿¹Á¦ÀÇ 2-fold dilution, 5 point´Â 98%ÀÇ È¿À²À» º¸¿©ÁÖ¾ú½À´Ï´Ù. Unknown sampleÀÇ ¿¹»ó°ª°ú °è»ê°ª »çÀÌ´Â 5% À̳»ÀÇ Á¤È®¼ºÀ» º¸¿©ÁÖ¾ú½À´Ï´Ù.
![MIC Magnetic Induction Cycler](https://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/1_5.png)
Relative Quantification
RQ ¼ÒÇÁÆ®¿þ¾î´Â ÃֽŠ¼öÇÐÀû ¸ðµ¨°ú ±Ù°Å¸¦ °®Ãá Åë°è ºÐ¼®À» »ç¿ëÇÏ¿©, ¿©·¯ ±×·ì¿¡¼ ¼·Î ´Ù¸¥ Ÿ°ÙÀÇ gene __________expression__________ level À» ºñ±³ÇÒ ¼ö ÀÖ½À´Ï´Ù. ÇÊ¿äÇÑ ¸ðµç °è»ê ¹× Åë°è´Â ¼ÒÇÁÆ®¿þ¾î ³»¿¡¼ ¼öÇàµË´Ï´Ù. µ¥ÀÌÅÍ´Â ¼ýÀÚ¿Í ±×·¡ÇÈÀ¸·Î È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. MicÀÇ Å¹¿ùÇÑ ¿Âµµ ±ÕÀϼºÀ¸·Î 0.2 fold ÀÇ ÀÛÀº »ùÇà °£ÀÇ Â÷À̸¦ ½±°Ô °¨Áö ÇÒ ¼ö ÀÖ½À´Ï´Ù.
![MIC Magnetic Induction Cycler](https://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/1_6.png)
Melt / Allelic Discrimination
FRET hybridization probe, beacon, Plexor µî ´Ù¾çÇÑ chemistries¿Í ºÐ¸®µÈ melt curves¸¦ »ç¿ëÇÏ¿© genotypeÀ» ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù. Melt peaks´Â ¹ÝÀü½Ãų ¼ö ÀÖ½À´Ï´Ù. Hybridization probe°ú Allelic DiscriminationÀ» ÀÌ¿ëÇÏ¿© genotypes¸¦ °áÁ¤ÇÒ ¼öµµ ÀÖ½À´Ï´Ù.
![MIC Magnetic Induction Cycler](https://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/1_7.png)
High Resolution Melting
HRM(¿É¼Ç) ¼ÒÇÁÆ®¿þ¾î´Â ±î´Ù·Î¿î Class IV SNP¸¦ Æ÷ÇÔÇÑ mutationÀ» ±¸ºÐÇÒ ¼ö ÀÖ½À´Ï´Ù. HRM is the perfect tool for applications including determining allele prevalence, screening for loss of heterozygosity, DNA fingerprinting, DNA methylation, species identification and calculating the ratio of somatic acquired mutations.
![MIC Magnetic Induction Cycler](https://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/1_8.png)
Specification
![MIC Magnetic Induction Cycler](http://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/2_1.jpg)
Front
![MIC Magnetic Induction Cycler](http://philekorea.kr/default/img/_images/list_img/mp12/sub2/list1/2_2.jpg)
Back
Physical
Height | 130mm (265mm lid open) |
Width | 150mm |
Length | 150mm |
Weight | 2 kg |
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Thermal Performance
Temperature Accuracy | ¡¾0.25¡ÆC |
Temperature Uniformity | ¡¾0.05¡ÆC (MIC-4+HRM) ¡¾0.10¡ÆC (MIC-2 and MIC-4) |
Ramp Rates | Heating 5¡ÆC/s Cooling 4¡ÆC/s |
Temperature Input Range | 35 – 99¡ÆC |
Optical
Detectors | Photodiode per channel | ||
Excitation Sources | High power LED for each channel | ||
Channels | Green | Ex 465nm | Em 510nm |
Yellow | Ex 540nm | Em 570nm | |
Orange | Ex 585nm | Em 618nm | |
Red | Ex 635nm | Em 675nm | |
Acquisition Time | 1 second |
Reaction Tubes
Samples per Instrument | 48 |
Reaction Volume Range | 5 – 30µL |
Communication
Type | USB 2.0 or Bluetooth |
PC Requirements | Windows 8 (32 bit) OS, Intel i5 2.4 GHz or equivalent processor, 4GB RAM, 1GB HD, .NET Framework 4.5. |
Operating Environment
Temperature | 18 – 30¨¬C |
Relative Humidity | 20 – 80% |
Product Information
Cat No. | Ç°¸í | ±Ô°Ý | Á¦Á¶»ç | Á¦Ç°Á¤º¸ |
---|---|---|---|---|
MIC-2 | MIC-2 Real time PCR | - | Bio molecular systems | - 2 Channel Green and Yellow. Standard Software - ¡¾0.1¡É uniformity |
MIC-4 | MIC-4 Real time PCR | - | Bio molecular systems | - 4 Channel Green, Yellow, Orange and Red , Standard Software - ¡¾0.1¡É uniformity |
MIC-4 + HRM | MIC-4 Real time PCR with HRM | - | Bio molecular systems | - 4 Channel Green, Yellow, Orange and Red. Standard Software - ¡¾0.1¡É uniformity |