qPCR Cycler
:MIC Magnetic Induction Cycler
MIC´Â ¼¼°è ÃÖÃÊ Magnetic Induction ¹æ½ÄÀÇ Real-time PCR ÀÔ´Ï´Ù. ºü¸£°í Á¤È®ÇÑ qPCR °á°ú¸¦ º¸ÀåÇÕ´Ï´Ù.
2kgÀÇ ÀÛÀº Å¥ºê ¾È¿¡ ´ã°ÜÁø °·ÂÇÑ ±â¼úÀ» °æÇèÇØ º¸¼¼¿ä.
Feature
- 1
¡¤ Magnetic Induction Technology¸¦ ÀÌ¿ëÇÑ ¿È¿À² ±Ø´ëÈ
¡¤ Channel º° µ¶¸³µÈ high intensity LED ¹× photodetector
¡¤ °íÁ¤µÈ optic module·Î calibration ¹× align ºÒÇÊ¿ä
¡¤ Rotor ¹æ½ÄÀÇ sample ȸÀüÀ¸·Î acquisition time 1ÃÊ ¼Ò¿ä
¡¤ ¿¹¿ ½Ã°£ÀÌ ÇÊ¿ä¾ø¾î ³¶ºñÇÏ´Â ½Ã°£ ¾øÀ½ - 2
¡¤ ȸÀüÇüÀÇ ¾Ë·ç¹Ì´½ ·ÎÅÍ·Î Á¤Àû / µ¿Àû ¿Âµµ±ÕÀϼº Á¦°ø
¡¤ ¿Âµµ Á¤È®¼º accuracy of ¡¾ 0.25 ¨¬C
¡¤ Well °£ ¿Âµµ ±ÕÀϼº uniformity of
¡¾ 0.05 ¨¬C maximum (MIC-4+HRM) ¡¾ 0.1 ¨¬C maximum (MIC-2 and MIC-4)
¡¤ HRMÀ» ÀÌ¿ëÇÑ Class IV SNP genotypingÀ» ½±°Ô ºÐ·ù - 3
¡¤ Bluetooth ¶Ç´Â USB ´ÙÁß¿¬°á °¡´É (ÃÖ´ë 10´ë)
¡¤ 48 - 96 - 192 - 480 ±îÁö È®À强 ¿ëÀÌ
¡¤ °³º° µ¥ÀÌÅÍÀÇ ÅëÇÕ ºÐ¼® °¡´É (ÃÖ´ë 480 samples)
¡¤ ¿ÀÇÂÇü ¼ÒÇÁÆ®¿þ¾î (Freeware) - 4
¡¤ ¼Õ¾È¿¡ µé¾î¿À´Â ÀÛÀº qPCR machine
¡¤ °¡·Î x ¼¼·Î x ³ôÀÌ / ¹«°Ô : 15 cm x 15 cm x 13 cm / 2 kg
¡¤ °íÁ¤µÈ optic module·Î calibration ¹× align ºÒÇÊ¿ä
¡¤ 0.1 §¢ qPCR Æ©ºê¸¦ ÀÌ¿ëÇÏ¿© total volume 5~30 §¡ °¡´É
¡¤ 4-strip tubeÀÇ °³º° rack Æ÷ÀåÀ¸·Î multi pipette ºÐÁÖ°¡´É
Application
Absolute Quantification
Standard curve¸¦ »ç¿ëÇϸé, À¯ÀüÀÚÀÇ Àý´ë·®À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. ¿¹Á¦ÀÇ 2-fold dilution, 5 point´Â 98%ÀÇ È¿À²À» º¸¿©ÁÖ¾ú½À´Ï´Ù. Unknown sampleÀÇ ¿¹»ó°ª°ú °è»ê°ª »çÀÌ´Â 5% À̳»ÀÇ Á¤È®¼ºÀ» º¸¿©ÁÖ¾ú½À´Ï´Ù.
Relative Quantification
RQ ¼ÒÇÁÆ®¿þ¾î´Â ÃֽŠ¼öÇÐÀû ¸ðµ¨°ú ±Ù°Å¸¦ °®Ãá Åë°è ºÐ¼®À» »ç¿ëÇÏ¿©, ¿©·¯ ±×·ì¿¡¼ ¼·Î ´Ù¸¥ Ÿ°ÙÀÇ gene __________expression__________ level À» ºñ±³ÇÒ ¼ö ÀÖ½À´Ï´Ù. ÇÊ¿äÇÑ ¸ðµç °è»ê ¹× Åë°è´Â ¼ÒÇÁÆ®¿þ¾î ³»¿¡¼ ¼öÇàµË´Ï´Ù. µ¥ÀÌÅÍ´Â ¼ýÀÚ¿Í ±×·¡ÇÈÀ¸·Î È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. MicÀÇ Å¹¿ùÇÑ ¿Âµµ ±ÕÀϼºÀ¸·Î 0.2 fold ÀÇ ÀÛÀº »ùÇà °£ÀÇ Â÷À̸¦ ½±°Ô °¨Áö ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Melt / Allelic Discrimination
FRET hybridization probe, beacon, Plexor µî ´Ù¾çÇÑ chemistries¿Í ºÐ¸®µÈ melt curves¸¦ »ç¿ëÇÏ¿© genotypeÀ» ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù. Melt peaks´Â ¹ÝÀü½Ãų ¼ö ÀÖ½À´Ï´Ù. Hybridization probe°ú Allelic DiscriminationÀ» ÀÌ¿ëÇÏ¿© genotypes¸¦ °áÁ¤ÇÒ ¼öµµ ÀÖ½À´Ï´Ù.
High Resolution Melting
HRM(¿É¼Ç) ¼ÒÇÁÆ®¿þ¾î´Â ±î´Ù·Î¿î Class IV SNP¸¦ Æ÷ÇÔÇÑ mutationÀ» ±¸ºÐÇÒ ¼ö ÀÖ½À´Ï´Ù. HRM is the perfect tool for applications including determining allele prevalence, screening for loss of heterozygosity, DNA fingerprinting, DNA methylation, species identification and calculating the ratio of somatic acquired mutations.
Specification
Front
Back
Physical
Height | 130mm (265mm lid open) |
Width | 150mm |
Length | 150mm |
Weight | 2 kg |
Thermal Performance
Temperature Accuracy | ¡¾0.25¡ÆC |
Temperature Uniformity | ¡¾0.05¡ÆC (MIC-4+HRM) ¡¾0.10¡ÆC (MIC-2 and MIC-4) |
Ramp Rates | Heating 5¡ÆC/s Cooling 4¡ÆC/s |
Temperature Input Range | 35 – 99¡ÆC |
Optical
Detectors | Photodiode per channel | ||
Excitation Sources | High power LED for each channel | ||
Channels | Green | Ex 465nm | Em 510nm |
Yellow | Ex 540nm | Em 570nm | |
Orange | Ex 585nm | Em 618nm | |
Red | Ex 635nm | Em 675nm | |
Acquisition Time | 1 second |
Reaction Tubes
Samples per Instrument | 48 |
Reaction Volume Range | 5 – 30µL |
Communication
Type | USB 2.0 or Bluetooth |
PC Requirements | Windows 8 (32 bit) OS, Intel i5 2.4 GHz or equivalent processor, 4GB RAM, 1GB HD, .NET Framework 4.5. |
Operating Environment
Temperature | 18 – 30¨¬C |
Relative Humidity | 20 – 80% |
Product Information
Cat No. | Ç°¸í | ±Ô°Ý | Á¦Á¶»ç | Á¦Ç°Á¤º¸ |
---|---|---|---|---|
MIC-2 | MIC-2 Real time PCR | - | Bio molecular systems | - 2 Channel Green and Yellow. Standard Software - ¡¾0.1¡É uniformity |
MIC-4 | MIC-4 Real time PCR | - | Bio molecular systems | - 4 Channel Green, Yellow, Orange and Red , Standard Software - ¡¾0.1¡É uniformity |
MIC-4 + HRM | MIC-4 Real time PCR with HRM | - | Bio molecular systems | - 4 Channel Green, Yellow, Orange and Red. Standard Software - ¡¾0.1¡É uniformity |