NEBNext® RNA Depletion Core Reagent Set
Feature
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.
The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.
· Compatible with a broad range of input amounts: 10 ng - 1µg
· Suitable for low-quality or high-quality RNA
· Fast workflow: 2 hours, with less than 10 minutes hands-on time
The kit is also available with RNAClean® beads.
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Customizable option to deplete unwanted RNA from any organism, using probe sequences designed with the NEBNext Custom RNA Depletion Design Tool.
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Compatible with a broad range of input amounts: 10 ng - 1 μg
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Suitable for low-quality or high-quality RNA
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Fast workflow: 2 hours, with less than 10 minutes hands-on time
