nCounter Analysis System

One Powerful, Fully Automated System for Translation Research
The nCounter® platform provides a simple and cost-effective solution for multiplex analysis of up to 800 RNA, DNA, or protein targets. Accelerate your research with just 15 minutes total hands-on time without amplification, cDNA conversion, or library prep and generate publication ready figures in ~24 hours. Combine the nCounter Analysis System with the GeoMx® Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Feature
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Save time
• Expertly curated pre-formatted panels for human, mouse, and other species
• 15 minutes total hands-on time with no amplification, cDNA conversion, or library prep required
• Sample to Publication ready figures in ~24 hours
• Analyze multiple pathways for up to 800 genes
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Save Sample
• Combine RNA, DNA, and protein panels for a comprehensive 3D Biology™ view of each sample
• Optimized performance on difficult sample types including FFPE, tissue, lysates, and biofluid samples
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Save Resources
• Advanced analysis tools included with system reduce the need for Bioinformatics support
• Digital gene expression eliminates need for technical replicates
How it works
The nCounter Analysis System features a streamlined workflow with a limited number of steps, improving the reliability of results and reducing the amount of time spent in the lab.
Specification
PLATFORM SPECIFICATION
| Feature | Specification | |
| Level of multiplexing | 800+ targets | |
| Recommended amount of starting material (Dependent on assay and sample type) |
RNA: 1ng-100ng DNA: 5ng-300ng |
|
| Sample types supported | Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene-lysed whole blood | |
| Reaction Volume | Up to 30 µl | |
| Limit of detection 0.5 fM spike-in control | 15 zeptomole spike-in control in 15µL hybridization | |
| Fold change sensitivity | > 1.5-fold (if > 5 copies per cell) > 2-fold (if > 1 copy per cell) R² ≥ 0.95) |
|
| Spike correlation | R2 ≥ 0.95 | |
| Linearity | Linear regression correlation coefficient R2 > 0.95 | |
| Linear dynamic range | 6 x 105 total counts | |
| Prep Station throughput | 12 lanes per 2.5 hours | |
| Digital Analyzer throughput | 12 samples per 2.7 hours | |
| Controls | Assay dependent | |

