nCounter Analysis System
One Powerful, Fully Automated System for Translation Research
The nCounter¢ç platform provides a simple and cost-effective solution for multiplex analysis of up to 800 RNA, DNA, or protein targets. Accelerate your research with just 15 minutes total hands-on time without amplification, cDNA conversion, or library prep and generate publication ready figures in ~24 hours. Combine the nCounter Analysis System with the GeoMx¢ç Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Feature
- 1
Save time
• Expertly curated pre-formatted panels for human, mouse, and other species
• 15 minutes total hands-on time with no amplification, cDNA conversion, or library prep required
• Sample to Publication ready figures in ~24 hours
• Analyze multiple pathways for up to 800 genes
- 2
Save Sample
• Combine RNA, DNA, and protein panels for a comprehensive 3D Biology¢â view of each sample
• Optimized performance on difficult sample types including FFPE, tissue, lysates, and biofluid samples
- 3
Save Resources
• Advanced analysis tools included with system reduce the need for Bioinformatics support
• Digital gene ____________expression____________ eliminates need for technical replicates
Molecules That Count¢ç
The nCounter Analysis System utilizes a novel digital color-coded barcode technology
hybridization
The nCounter Analysis System utilizes a novel digital color-coded barcode technology
PURIFY and IMMOBILIZE(Sample Processing)
The nCounter Analysis System utilizes a novel digital color-coded barcode technology
Specification
PLATFORM SPECIFICATION
Level of multiplexing | Up to 800 nucleic acid targets per assay | |
Recommended amount of starting material | 100 ng of total RNA, or lysate from ~10,000 cells | |
Sample types supported | Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene lysed whole blood | |
Limit of detection | 15 zeptomole spike-in control | |
Fold change sensitivity | 1.5 fold (> 5 copies per cell); > 2 fold change (> 1 copy per cell) | |
Spike correlation | R2 ¡Ã 0.95 | |
Linear dynamic range | 7 x 105 total counts | |
Throughput | nCounter SPRINT | nCounter MAX/FLEX |
12 lanes per 6 hours | 12 lanes per 2.5 hours | |
12 samples per 2.7 hours |