EZ-Tn5 Transposome Kits
Features
- 1
Generate random gene knockouts in living bacteria
- 2
Generate mutants with improved genetics or function across a broad host range of bacterial cell types
- 3
Characterise novel genes and gene functions
- 4
Identify genes involved in pathogenesis, toxicity, biofilm development
- 5
Unravel metabolic pathways
- 6
Identify essential genes and regulatory elements
- 7
100s of citations for many different applications
Product information
EZ-Tn5¢â Transposome¢â complexes are formed between an EZ-Tn5 Transposon and EZ-Tn5 Transposase, and provide a simple and reliable method for generating a library of random gene knockouts in vivo.* Just electroporate the EZ-Tn5 Transposome into any of a broad range of living bacterial cells and select for a marker encoded by the EZ-Tn5 Transposon (Figure 1.) Because there is no need for cell conjugation, suicide vectors, or specific host factors, EZ-Tn5 Transposomes are ideal for creating mutants in species that have poorly described genetic systems or lack adequate molecular tools.