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Pichia Expression System

사용하기 쉽고, 강력한 Pichia expression system으로, 재조합 단백질을 Yeast에서 발현하는 키트.
한국생명공학연구원과 함께 R&D 진행, 개발 중

Lab Service / R&D> Pichia Expression System

Research & Development Center

APExpress™ Pichia expression vector

프로모터 : 상시발현 프로모터 (Pichia pastoris TEF promoter) 활용

분비서열 : 단백질 분비를 위한 Signal Peptide 서열

숙주세포 : 야생형 균주 게놈 편집을 통한 단백질 고효율 발현용 균주를 이용

*PpTEF promoter, Pichia pastoris translational elongation factor EF-1 alpha promoter.

Features for AP-Pp-1 3827 base pairs

Target genome site: bases 18-683

PpTEF* promoter: bases 712-1148

Codon-optimized MR(KR): bases 1149-1403

SapI: bases 1404-1410, 1463-1469

3’-UTR: bases 1474-1547

AOX1 terminator: bases 1548-1794

FRT (minimal): bases 1796-1829

TEF1 Promoter: bases 1847-2244

EM7 promoter: bases 2251-2298

Selection marker: bases 2317-2691

PpGAPDH terminator: bases 2698-3190

pBR322 origin: bases 3199-3787

FRT (minimal): bases 1796-1829, 3794-3827

Type II 제한효소 (Sap I) 사용으로 Target gene Assembly 가능

Target genome site를 적용한 Homologous recombination 가능

TEF-EM7 Hybrid promoter 도입으로 E. coli 와 Yeast (P. pastoris)에서 동일 selection marker (e.g. BleoR) 적용가능

APExpress™ Pichia Expression vector는 재조합 단백질을 yeast Pichia pastoris에서 높은 수준으로 발현 가능

APExpress™ Pichia Expression Kit는 PpTEF promoter에서 목적 유전자를 발현시키기 위해 필수적인 구성품을 제공

APExpress™ Pichia expression vector

Promotor : Glucose regulated constitutive TEF promoter(Pichia pastoris)

Signalpeptide : P. pastoris mating factor α (MFα) for secreted expression from Saccharomyces cerevisiae.

Host strain : Genome editing of wild-type strains for high-efficiency protein expression

*PpTEF promoter, Pichia pastoris translational elongation factor EF-1 alpha promoter.

Features for AP-Pp-1 3827 base pairs

Target genome site: bases 18-683

PpTEF* promoter: bases 712-1148

Codon-optimized MR(KR): bases 1149-1403

SapI: bases 1404-1410, 1463-1469

3’-UTR: bases 1474-1547

AOX1 terminator: bases 1548-1794

FRT (minimal): bases 1796-1829

TEF1 Promoter: bases 1847-2244

EM7 promoter: bases 2251-2298

Selection marker: bases 2317-2691

PpGAPDH terminator: bases 2698-3190

pBR322 origin: bases 3199-3787

FRT (minimal): bases 1796-1829, 3794-3827

Type II restriction enzyme(SapI) in the multiple cloning site for target gene assembly

TEF-EM7 Hybrid promoter for select-able marker(e. g. BleoR) application both E. coli and yeast (P. pastoris).

APExpress™ pichia Expression kit is manufactured for high yield recombinant protein expression in yeast Pichia pastoris

APExpress™ pichia Expression kit is supplied all component for high level expression of recombinant proteins

The AOX1 promoter is used to drive expression of the gene of interest encoding the desired heterologous protein in APExpress™ pichia Expression System.