DNA Assembly

The successful assembly of DNA can be utilized for both cloning and synthetic biology (in which genes and proteins are redesigned and/or assembled into novel ways to create new and useful functionality). New England Biolabs offers several products that can be used for DNA assembly and cloning of multiple DNA fragments, including NEB Gibson Assembly®, NEBuilder® HiFi DNA Assembly, which utilizes a higher fidelity polymerase, and Golden Gate Assembly. Please refer to the DNA Assembly Selection Chart to determine which product would work best for your needs.

PCR Cloning

Fluorescence based recombinant systems are attractive molecular imaging tools because of their advantages for in vivo studies. Both bioluminescence reporters and fluorescence labeling systems offer relatively easy transition from in vitro, to living cells, tissues or small whole animal analysis (2). These protein labeling and reporter systems are non-invasive and allow direct analysis of cellular and molecular dynamics in real time. Some examples of bioluminescent reporters useful for in vivo imaging are enhanced GFP, FAP, Gaussia Luciferase (GLuc) and Cypridina Luciferase (Cluc). Protein labeling systems such as Tetra-Cys, SNAP-tag® and CLIP-tag™ can also be used for in vivo techniques. Imaging in living animals is currently best accomplished by PET reporters for scanning with short-lived radioactive tracers. Multimodality reporter gene systems have also been designed to permit in vivo imaging by multiple detection methods (3). For any fluorescence imaging application, fluorophore selection for native target activities and photostability should be confirmed in heterogeneous cellular environments.

Site-Directed Mutagenesis

Fluorescence based recombinant systems are attractive molecular imaging tools because of their advantages for in vivo studies. Both bioluminescence reporters and fluorescence labeling systems offer relatively easy transition from in vitro, to living cells, tissues or small whole animal analysis (2). These protein labeling and reporter systems are non-invasive and allow direct analysis of cellular and molecular dynamics in real time. Some examples of bioluminescent reporters useful for in vivo imaging are enhanced GFP, FAP, Gaussia Luciferase (GLuc) and Cypridina Luciferase (Cluc). Protein labeling systems such as Tetra-Cys, SNAP-tag® and CLIP-tag™ can also be used for in vivo techniques. Imaging in living animals is currently best accomplished by PET reporters for scanning with short-lived radioactive tracers. Multimodality reporter gene systems have also been designed to permit in vivo imaging by multiple detection methods (3). For any fluorescence imaging application, fluorophore selection for native target activities and photostability should be confirmed in heterogeneous cellular environments.

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