Genome Editing
Feature
New England Biolabs provides reagents to support a broad variety of CRISPR/Cas9 genome editing approaches. From introduction of Cas9 and single guide RNA (sgRNA) on plasmids, to direct introduction of Cas9 ribonucleoprotein (RNP) and detection of edits using next generation sequencing or enzymatic mutation detection, NEB provides reagents that simplify and shorten genome editing workflows.
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EnGen® Spy Cas9 NLS, EnGen Lba Cas12a, EnGen Sau Cas9: for genome engineering by direct introduction of active nuclease complexes
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EnGen® sgRNA Synthesis Kit: for rapid generation of microgram quantities of custom sgRNA
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EnGen® Mutation Detection Kit: for robust determination of targeting efficiency
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EnGen® Spy Cas9 Nickase: programmable site-specific DNA nicking for genome editing and in vitro applications
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EnGen Spy dCas9 (SNAP-tag): Programmable DNA binding activity compatible with broad variety of SNAP-tag conjugates
