High-quality stable cell line generation from LentiORF Select-Vectors™

Transomic offers over__expression__ stable cell lines generated from LentiORF Select-Vectors^TM. Our extensive collection of constitutive and inducible lentiviral vectors allows for customization of the target gene __expression__ cassette to include the appropriate selectable markers and tags for the specific experiment.

Customizable lentiviral and retroviral vectors to fit your specific research need

Let Transomic engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.

Create the ideal ORF __expression__ vector for your experiment

Use Transomic’s gene synthesis service for creating the ideal lentiviral ORF __expression__ vector for your experiment. Transomic offers solutions whether you need the wild-type sequence, an isoform, a mutant, or an optimized design for a rescue experiment. Just send us the sequence you would like to have synthesized or the gene name/accession number and we will codon optimize your gene for efficient synthesis. We will deliver your gene in the ideal LentiORF Select-Vector as a glycerol stock, plasmid DNA, or ready-to-use lentiviral particles.

Custom Next-Generation Sequencing and Deconvolution Services

Transomic can help analyze results from your CRISPR pooled screening experiments. Send us cell pellets from your screen and we will extract genomic DNA, prepare the samples for Next-Generation Sequencing (NGS), perform NGS on the appropriate NGS platform, and align the gRNA sequence reads against the NGS reads from the screening pool. You can then determine which gRNAs in your screening pool were enriched or depleted.

Highest quality Lentiviral packaging Service

Transomic offers the highest quality lentiviral packaging services using second and/or third generation packaging plasmids that have been optimized to produce higher viral titers compared to commercially available packaging plasmids. Additionally, we understand the importance of accurate viral titer counts and provide functional titer measurements for your packaged particles. This method is a much more reliable measurement compared to simply measuring viral particle levels.

Choose constructs from our genome-wide arrayed CRISPR library or chip-based pools for complete flexibility

With an extensive catalog of pCLIP lentiviral vectors to choose from, Transomic offers complete flexibility when planning your custom CRISPR pooled screening library. Share your target gene list with us and we will use ourCRoatan algorithmto design gRNAs targeting each gene. Or if preferred, share your gRNA designs to incorporate into the screening pool.

Choose constructs from our genome-wide arrayed shRNA library or chip-based pools for complete flexibility

With an extensive catalog of pZIP lentiviral vectors to choose from, Transomic offers complete flexibility when planning your custom RNAi screen. Share your target gene list with us and we will use ourshERWOOD algorithmto design shRNAs targeting each gene. Or if preferred, share your shRNA designs to incorporate into the screening pool.

Choose constructs from our genome-wide arrayed CRISPR library or chip-based pools for complete flexibility

Let Transomic build your custom CRISPR arrays using any of our pCLIP lentiviral vectors. Share your target gene list with us and we will use our CRoatan algorithm to design gRNAs targeting each gene. Or if preferred, share your gRNA designs for cloning into our lentiviral vector, or a lentiviral vector that you provide to us. Each clone is sequence-verified and arrayed into 96-well plates. We can deliver glycerol stocks, plasmid DNA, and/or ready-to-use lentiviral particles in 96-well plates.

Choose constructs from our genome-wide arrayed shRNA library or chip-based pools for complete flexibility

Let Transomic build your custom shRNA arrays using any of our pZIP lentiviral vectors. Share your target gene list with us and we will use our shERWOOD algorithm to design shRNAs targeting each gene. Or if preferred, share your shRNA designs for cloning into our lentiviral vector, or a lentiviral vector that you provide to us. Each clone is sequence-verified and arrayed into 96-well plates. We can deliver glycerol stocks, plasmid DNA, and/or ready-to-use lentiviral particles in 96-well plates.