NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®
Feature
The NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
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The highest yields of high-quality sequencing libraries from single cells, or 2 pg - 200 ng RNA
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Input can be cultured or primary cells, or total RNA
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Low abundance transcripts are easily detected
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High quality libraries and sequence data are generated for a wide range input amounts and sample types
· Full length transcript coverage
· Consistent transcript detection
· Superior transcript correlation - 5
Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time
· Single-tube protocol from cell lysis to cDNA
· DNA fragmentation, end repair and dA-tailing reagents in a single enzyme mix, with a single protocol, regardless of GC content
