QuickExtract FFPE DNA Extraction Kit

Features
- 1
Simple, rapid extraction of PCR-ready DNA from challenging FFPE samples
- 2
Fast:
PCR-ready DNA in minutes, not days
- 3
Simple:
No spin columns or transfer steps which helps increase yields
- 4
Compatible:
PCR-ready DNA in minutes, not days
- 5
Safe:
PCR-ready DNA in minutes, not days
Product information
The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process. These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.
The QuickExtract¢â FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples (Figure. 1). The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR (Figures. 2 and 3).