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nanoString Multiplexed gene expression analysis > nCounter System > nCounter System

nCounter System

1. nCounter® Gene Expression CodeSets

nCounter® Gene Expression CodeSets offer a cost-effective way to analyze the expression levels of up to 800 genes simultaneously, with high precision and accuracy. The system can directly assay tissue and blood lysates as well as Formalin-Fixed Paraffin-Embedded (FFPE) extracts in a simple workflow that requires only 15 minutes of total hands-on time per reaction.

Feature

Gene Expression Panels

Many off-the-shelf Panel Kits are currently available which include CodeSets targeting genes associated with inflammation, immunology, and cancer as well as a reference gene CodeSet  for aiding in reference gene selection. Each Panel Kit arrives in your lab QC’d and ready-to-use with all the reagents and consumables you need to perform the assay.

  1. 1. nCounter® GX Human Kinase Kit

    : Gene Expression CodeSet profiling of 519 human kinase genes and 8 internal reference genes. CodeSets are available  in quantities to run 12, 24, 48 or 96 assays

  2. 2. nCounter® GX Human Immunology Kit

    : Gene Expression CodeSet profiling 511 immunology-related human genes and 15 internal reference genes. CodeSets  are available in quantities to run 12, 24, 48 or 96 assays

  3. 3. nCounter® Human Cancer Reference Kit

    : Gene Expression CodeSet profiling 230 cancer-related human genes and 6 internal reference genes. CodeSets are  available in quantities to run 12, 24, 48 or 96 assays.

  4. 4. nCounter® Human Inflammation Kit

    : Gene Expression CodeSet profiling 184 inflammation-related human genes and 6 internal reference genes. CodeSets  are available in quantities to run 12, 24, 48 or 96 assays

  5. 5. nCounter® Mouse Inflammation Kit

    : Gene Expression CodeSet profiling 179 inflammation-related mouse genes and 6 internal reference genes. CodeSets  are available in quantities to run 12, 24, 48 or 96 assays

  6. 6. nCounter® Human Reference GX Kit

    : Gene Expression CodeSet profiling 18 human reference genes expressed at different levels across a wide variety of  tissues. This kit is designed to provide a fast and easy way to select reference genes for your experiments. CodeSets are  available in quantities to run 12, 24, 48 or 96 assays

  7. 7. nCounter® Customer Assay Evaluation (CAE) Kit

    : Gene Expression CodeSet profiling 48 human genes from the Microarray Quality Control Study (MAQC). This kit is  designed for core labs to train additional operators of the nCounter Analysis System. CodeSets are available in quantities  to run 12, 24, 48 or 96 assays

  8. 8. nCounter® Virtual Gene Sets

  9. 9. Oncology Panel

    •  ① PanCancer Pathways Panel        Chromatin Modification, and TGF-ẞ.
    • ② PanCancer Immune Profiling Panel
    •  ③ PanCancer Progression Panel        metastasis.
    •  ④ Breast Cancer 360™ Panel        Inflammation Signature* (TIS) assays
    •  ⑤ PanCancer IO 360™ Panel        described in JCI

  10. 10. Neuroscience Panel

    •   ① Neuropathology Panels        neuroinflammation and metabolism.
    •   ② Neuroinflammation Panels        inflammation, neurobiology and neuropathology, and metabolism and stress

Custom CodeSets

NanoString also offers the ultimate in flexibility by designing CodeSets to your specifications for any organism and gene set that you wish to analyze. Each customer receives a free consultation with our CodeSet design team.

Sensitivity and Reproducibility

Sensitivity, Reproductibility and Dynamic Range

Gene counts from two technical replicates plotted against one another demonstrate the assay reproducibility over a wide dynamic range (10–50,000 counts).  One total RNA sample was split into two separate hybridization reactions and processed independently on the nCounter Analysis System. In this experiment, 75 counts is equal to a concentration of approximately 1 copy per cell. This data illustrates the high level of sensitivity and precision of the assay even at very low levels of expression.

Cell Lysate with Matched Total RNA

Raw cell lysate was input directly into the nCounter Analysis System hybridization reaction using approximately 2,500, 5,000 and 10,000 cells in lysis buffer and compared to 100ng of purified total RNA as plotted on the x-axis. Results were highly correlated with one another and demonstrate that comparable data can be achieved with either protocol.

Sample Flexibility

Matched Whole Blood Lysate and Total RNA

PAXgene™ lysed whole blood replicates were input directly into the hybridization reaction and compared to a matched purified total RNA sample as plotted on the x-axis. Results demonstrate that high quality data can be obtained by using PAXgene lysed whole blood.

FFPE Samples

FFPE-derived purified total RNA was input directly into the hybridization reaction and compared to a matched purified total RNA from fresh tissue on the x-axis. Results demonstrate that high quality data can be achieved from FFPE samples.



nCounter Analysis System


Overview of the nCounter Analysis System and critical performance specifications.

nCounter Single Cell Gene Expression Assay

Analyze multiple pathways for up to 800 genes, eliminate sample splitting, minimize amplification - get better data from every cell.

nCounter Plex2 Assay

Analyze expression levels in multiple samples and hundreds of gene targets simultaneously in a single tube.

nCounter v2 Cancer CN Assay

Copy number analysis of 87 genes commonly amplified or deleted in cancer.

nCounter Human v2 miRNA Expression Assay

Overview of the nCounter Human v2 miRNA Expression Assay Kit, an off-the-shelf panel profiling of 800 human miRNAs from miRBase v.18.

nCounter Mouse miRNA Expression Assay

Overview of the nCounter Mouse miRNA Expression Assay Kit, an off-the-shelf panel profiling of more than 600 murine and murine-associated viral miRNAs.

nCounter Rat miRNA Expression Assay

Overview of the nCounter Rat miRNA Expression Assay Kit, an off-the-shelf panel profiling of 423 rat miRNAs.

nCounter D. melanogaster (Fly) miRNA Expression Assay

Overview of the nCounter Fly miRNA Expression Assay Kit, an off-the-shelf panel profiling of 184 D. melanogaster miRNAs.

nCounter miRGE Assay CodeSet

Overview of the nCounter miRGE Expression Assay Kit, a customized CodeSet which can measure between 5-30 miRNAs and 100-200 mRNAs in a single reaction.

nCounter Custom lncRNA Assay

Study focused sets of up to 800 lncRNAs in a single reaction.

nCounter ChIP-String Assay

Customized CodeSet for analysis of DNA enriched by chromatin immunoprecipitation.

nCounter Leukemia Fusion GX Assay

Overview of the nCounter Fusion Gene Expression Assay and critical performance specifications.

nCounter Copy Number Variation Assay

nCounter Human Karyotype

nCounter GX Human Immunology

Gene Expression CodeSet profiling 511 immunology-related human genes and 15 internal reference genes. CodeSets are available in quantities to run 12, 24, 48 or 96 assays.

nCounter GX Human Kinase

Overview of the nCounter GX Human Kinase Kit, an off-the-shelf panel enabling gene expression profiling of 519 human Kinase genes in a single reaction.

nCounter Mouse miRNA Expression Assay

Overview of the nCounter Mouse miRNA Expression Assay Kit, an off-the-shelf panel profiling of over 600 mouse and mouse-associated viral miRNAs derived from miRBase.

nCounter Rat miRNA Expression Assay

Overview of the nCounter Rat miRNA Expression Assay, an off-the-shelf panel profiling of 400 rat miRNAs in a single reaction.

nCounter Custom Copy Number Variation Assay (Early Access)

Overview of the nCounter Customer Copy Number Variation Assay Kit, a custom assay for human genomic DNA that allows users to analyze up to 200 genomic regions in a single multiplexed reaction.

nCounter Human Cancer Reference

Overview of the nCounter Human Cancer Reference Kit, an off-the-shelf panel enabling gene expression profiling of 230 cancer-related human genes in a single reaction.

nCounter Human Inflammation

Overview of the nCounter Human Inflammation Kit, an off-the-shelf panel enabling gene expression profiling of 184 inflammation-related human genes in a single reaction.

nCounter Mouse Inflammation

Overview of the nCounter Mouse Inflammation Kit, an off-the-shelf panel enabling gene expression profiling of 179 inflammation-related mouse genes in a single reaction.

nCounter Virtual Angiogenesis Gene Set

Pre-selected angiogenesis gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Breast Cancer-Estrogen Receptor Gene Set

Pre-selected breast cancer-estrogen receptor gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual p53 Pathway Gene Set

Pre-selected p53 pathway gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Apoptosis Gene Set

Pre-selected apoptosis gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Cell Cycle Gene Set

Pre-selected cell cycle gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Jak-Stat Pathway Gene Set

Pre-selected jak-stat pathway gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Stem Cell Gene Set

Pre-selected stem cell gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Wnt Pathway Gene Set

Pre-selected wnt pathway gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Mouse Cancer Gene Set

Pre-selected mouse cancer gene set to accelerate your research; Made to order, rapidly delivered.

nCounter Virtual Mouse Stem Cell Gene Set

Pre-selected mouse stem cell gene set to accelerate your research; Made to order, rapidly delivered.


nCounter GX Human Kinase Kit


nCounter Human Cancer Reference Gene Set


nCounter Human Reference GX Kit


nCounter Human Inflammation Kit Gene Set


nCounter Virtual Angiogenesis Gene Set


nCounter GX Human Immunology Kit Gene List


nCounter Mouse Inflammation Kit Gene Set


nCounter Virtual Breast Cancer-Estrogen Receptor Gene Set


nCounter Customer Assay Evaluation (CAE) Kit


nCounter Virtual Apoptosis Gene Set


nCounter Virtual Cell Cycle Gene Set


nCounter Virtual p53 Pathway Gene Set


nCounter Virtual Jak-Stat Pathway Gene Set


nCounter Virtual Stem Cell Gene Set


nCounter Virtual Wnt Pathway Gene Set


nCounter Virtual Mouse Cancer Gene Set


nCounter Virtual Mouse Stem Cell Gene Set


nCounter Human v2 miRNA Expression Assay Target List - NEW


nCounter Mouse miRNA Expression Assay Target List


nCounter Rat miRNA Expression Assay Target List


nCounter Fly miRNA Expression Assay Target List