Moxi¥´
Gold Standard Cell Counts, Cell Size, and Viability
The Moxi V provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Flow Cytometer) to provide the most accurate cell counts, size, and viability in the industry. Specifically, the Moxi V employs the Coulter Principle to precisely measure the volumetric particle size of each particle for exact size measurements down to 3um in diameter (14fL volume), easily distinguishing between cells and debris. The system is also equipped with a 532nm laser and a 561nm/LP detection channel for robust cell viability analysis. Propidium Iodide (PI)-stained dead cells measure 50-100 times brighter fluorescence on the system than do live cells, removing the ambiguity associated with traditional Trypan Blue viability assessments. For each test, these size and viability measurements are applied, individually, to up to 23,000 cells in a matter of a few seconds. This ensures the highest level of precision and statistical robustness. As the fluidic volume is precisely metered as well, the particle counts are presented as an exact cell concentration.
The Moxi V employs a patented, single-use, microfluidic flow cell. The flow cells eliminate the hassle of traditional flow cytometers and Coulter Counters, eliminating the need for cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes. The Moxi V uses very little sample volume, 60ul, allowing you to conserve your precious, potentially expensive, sample (e.g. stem cells). Cell concentrations as low as 10 cells/ul are possible, typically requiring just 5ul of cell sample diluted in 55ul of PBS.
The Moxi V¢â system comes standard with an ultra-intuitive, plug-and-play interface with free OS updates for as long as you own the instrument. No prior flow cytometry experience is required – you simply just plug and play.
Feature
- 1
True Cell Viability Counts - 50-100x more sensitive than vision counters.
- 2
Precision Sizing - Uses the Coulter Principle to get precise cell volumes with CVs less than 3% and no need for triplicates.
- 3
Highly Accurate - Accurate counts for smaller cells down to 3um (i.e. nuclei, RBCs). Uniquely accurate at low cell concentrations down to 10 cells per ul.
- 4
Rapid Assays - Offers a less than 15 second test that counts up to 23,000 cells compared to 200-300 counts on most vision counters.
How It Works
The operating principle behind the Moxi V is a unique combination of Coulter-Principle cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser with a 561nm/LP (e.g. PI) emission filter. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is automatically performed by the system but can be easily adjusted/tuned by the user. The resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
Step 1:
Select desired app, insert the cassette and close the doors.
Step 2:
Once auto-alignment is complete, open the top door and pipette 60¥ìl of labelled sample into the cassette.
Step 3:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi V, and load Sample 2.
Data:
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any flow cytometry analysis package. The actual Moxi V screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi V and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.
Specification
Moxi go V
id | MXV102 |
Included Accessories | USB power cord, US style USB power adapter, and Type S+ cassette pack |
AC Power Type | 110 VAC |
Applications | Gold Standard Cell Count and Viability |
Battery Type | Rechargeable 3.7 V, 7500 mAh lithium ion |
Cassette Types | Type S+ |
Cell Particle Concentration Range | 10,000 - 1,000,000 cells/mL Type S+ |
Cell Types Tested | HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S.cerevisiae (Baker's Yeast|Safale US-05 Yeast| |
Data Output Formats | FCS 3.1, screen shots (.bmp), CSV |
Data Storage Capacity | 4Gb |
Display Resolution | 800 x 480 color touchscreen |
Excitation Wavelengths | 532nm |
In British Units | 8 lbs |
Intended Use Statement | For Research Use Only. Product is not for use in diagnostic procedures |
Laser Color | Green |
Measurable Dynamic Range | 3 - 27 microns Type Type S+; 4 - 35 microns Type MF-M |
Measurement Time | 10 seconds Type S+ |
MPI Cell Health Ratio | Yes (Size histogram only) |
Number of Detection Channels Flow parameters | 1 color, 1 size, 1 forward extinction |
Number of PMTs | 1 |
Optical Detection Range | 525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP) |
Particle Size Detection Method | Impedimetric (Coulter Principle) |
Platform | Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein) |
Pre-Programmed Tests | Mulitplexed Bead ELISA's|In Cell Westerrns|In Cell Protein Quant|RFP|Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis |
Resolution Histogram Bins | 1000 |
Sample Type | Beads|Cell Preparations |
Sample Volume | 160 µL |
Supported Connectivity | USB on-the-go |
Useable Cell Volume | 14 - 10,306 fL Type S+; 14 - 22,449 fL Type MF-M |
Weight | 3.6kg |
Product information
Cat No. | Ç°¸í | ±Ô°Ý | Á¦Á¶»ç | Á¦Ç°Á¤º¸ |
---|---|---|---|---|
MXV102 | Moxi V Kit | 1 unit | ORFLO | - |