Epigenetics
New England Biolabs has called upon its 40 years of expertise in enzymology to develop several solutions for epigenetics research, including NEBNext¢ç Enzymatic Methyl-seq (EM-seq¢â) and the EpiMark¢ç suite of products.
A new method for DNA methylation analysis at the single base level, NEBNext Enzymatic Methyl-seq (NEB #E7120), is now available. This enzyme-based technology minimizes damage to DNA and produces high quality libraries that enable superior detection of 5mC and 5hmC from fewer sequencing reads.
Easy-to-use EpiMark kits simplify DNA methylation (5mC) and hydroxymethylation (5hmC) detection and analysis, as well as ChIP, histone and nucleosome analysis. Independently applicable, individual epigenetics reagents also complement the EpiMark¢ç kits. NEB's methylation- and hydroxymethylation- sensitive or dependent enzymes, DNA methyltransferases and DNA controls are all useful for mapping DNA modifications and methylating DNA at specific sites for gene __expression__ studies. Our protein methyltransferases and recombinant histones perform efficiently in protein modification and characterization studies. Our range of modified and unmodified genomic DNAs can be used as controls for detection of DNA methylation. Our series of human DNA (cytosine-5) methyltransferase (DNMT) antibodies are ideally suited for Western blots and immunoprecipitation.
Our complete Epigenetics suite is expertly designed for optimized research and discovery.
Specification
Antibodies
Control DNA
Epigenetic Analysis
Histones
Methylation Dependent Restriction Enzymes for Epigenetics
Methylation Sensitive Restriction Enzymes for Epigenetics
Methyltransferases for Epigenetics
Product Information
Epigenetics
Cat No. | SIze | |
---|---|---|
NEBNext¢ç Enzymatic Methyl-seq Conversion Module | E7125S | 24 reactions |
E7125L | 96 reactions | |
NEBNext¢ç Enzymatic Methyl-seq Kit | E7120S | 24 reactions |
E7120L | 96 reactions | |
NEBNext¢ç Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) | E7140S | 24 reactions |
E7140L | 96 reactions | |
NEBNext¢ç Q5U¢ç Master Mix | M0597S | 50 reactions |
M0597L | 250 reactions | |
Nucleoside Digestion Mix | M0649S | 50 reactions |
Antibodies | ||
50 ml Magnetic Separation Rack | S1507S | 4 tubes |
Anti-SNAP-tag¢ç Antibody (Polyclonal) | P9310S | 100 µl |
Protein A Magnetic Beads | S1425S | 1 ml |
Protein G Magnetic Beads | S1430S | 1 ml |
Control DNA | ||
Nucleosome Control DNA | N1202S | 0.2 nmol |
Epigenetic Analysis | ||
DpnI | R0176S | 1,000 units |
R0176L | 5,000 units | |
DpnII | R0543S | 1,000 units |
R0543T | 1,000 units | |
R0543L | 5,000 units | |
R0543M | 5,000 units | |
EpiMark¢ç 5-hmC and 5-mC Analysis Kit | E3317S | 20 reactions |
EpiMark¢ç Bisulfite Conversion Kit | M0490S | 100 reactions |
M0490L | 500 reactions | |
EpiMark¢ç Methylated DNA Enrichment Kit | E2600S | 25 reactions |
EpiMark¢ç Nucleosome Assembly Kit | E5350S | 20 reactions |
EpiMark¢ç Hot Start Taq DNA Polymerase | M0490S | 100 reactions |
M0490L | 500 reactions | |
HpaII | R0171S | 2,000 units |
R0171L | 10,000 units | |
R0171M | 10,000 units | |
LpnPI | R0663S | 200 units |
McrBC | M0272S | 500 units |
M0272L | 2,500 units | |
MspI | R0106S | 5,000 units |
R0106T | 5,000 units | |
R0106L | 25,000 units | |
R0106M | 25,000 units | |
NEBNext¢ç Enzymatic Methyl-seq Conversion Module | E7125S | 24 reactions |
E7125L | 96 reactions | |
NEBNext¢ç Enzymatic Methyl-seq Kit | E7120S | 24 reactions |
E7120L | 96 reactions | |
NEBNext¢ç Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) | E7140S | 24 reactions |
E7140L | 96 reactions | |
NEBNext¢ç Q5U¢ç Master Mix | M0597S | 50 reactions |
M0597L | 250 reactions | |
Nucleoside Digestion Mix | M0649S | 50 reactions |
T4 Phage ¥â-glucosyltransferase (T4-BGT) | M0357S | 500 units |
M0357L | 2,500 units | |
Histones | ||
EpiMark¢ç Nucleosome Assembly Kit | E5350S | 20 reactions |
Histone H10 Human, Recombinant | M2501S | 100 µg |
Histone H2A Human, Recombinant | M2502S | 100 µg |
Histone H2A/H2B Dimer Human, Recombinant | M2508S | 2 nmol |
Histone H2B Human, Recombinant | M2505S | 100 µg |
Histone H3.1 Human, Recombinant | M2503S | 100 µg |
Histone H3.1/H4 Tetramer Human, Recombinant | M2509S | 1 nmol |
Histone H3.2 Human, Recombinant | M2506S | 100 µg |
Histone H3.3 Human, Recombinant | M2507S | 100 µg |
Histone H4 Human, Recombinant | M2504S | 100 µg |
Methylation Dependent Restriction Enzymes for Epigenetics | ||
FspEI | R0662S | 200 units |
LpnPI | R0663S | 200 units |
McrBC | M0272S | 500 units |
M0272L | 2,500 units | |
MspJI | R0661S | 200 units |
R0661L | 1,000 units | |
Methylation Sensitive Restriction Enzymes for Epigenetics | ||
DpnII | R0543S | 1,000 units |
R0543T | 1,000 units | |
R0543L | 5,000 units | |
R0543M | 5,000 units | |
HpaII | R0171S | 2,000 units |
R0171L | 10,000 units | |
R0171M | 10,000 units | |
MspI | R0106S | 5,000 units |
R0106T | 5,000 units | |
R0106L | 25,000 units | |
R0106M | 25,000 units | |
SalI-HF¢ç | R3138S | 2,000 units |
R3138T | 2,000 units | |
R3138L | ||
R3138M | 10,000 units | |
ScrFI | R0110S | 1,000 units |
Methyltransferases for Epigenetics | ||
AluI Methyltransferase | M0220S | 100 units |
BamHI Methyltransferase | M0223S | 100 units |
CpG Methyltransferase (M.SssI) | M0226S | 100 units |
M0226L | 500 units | |
M0226M | 500 units | |
dam Methyltransferase | M0222S | 500 units |
M0222L | 2,500 units | |
EcoGII Methyltransferase | M0603S | 200 units |
EcoRI Methyltransferase | M0211S | 10,000 units |
GpC Methyltransferase (M.CviPI) | M0227S | 200 units |
M0227L | 1,000 units | |
HaeIII Methyltransferase | M0224S | 500 units |
Hhal Methyltransferase | M0217S | 1,000 units |
HpaII Methyltransferase | M0214S | 100 units |
MspI Methyltransferase | M0215S | 100 units |
TaqI Methyltransferase | M0219S | 1,000 units |