Label-free, Real-time, Molecular Interaction analysis system :WAVEsystem¢ç
feature
- 1
GCI (Grating-coupled Interferometry) ¿ø¸® ¹× Label-free ¹æ½ÄÀ¸·Î ½Ç½Ã°£ °áÇÕ ¹ÝÀÀ ÃøÁ¤
Creoptix»çÀÇ Creoptix ¢ç WAVEsystem¢çÀº ¶óº§ ÇÁ¸® »ùÇÃÀÇ ½Ç½Ã°£ ¹°Áú °áÇÕ¹ÝÀÀ ºÐ¼® (ģȵµ, Ä«À̳×ƽ½º) ÃøÁ¤À» °í°¨µµ, °í¼ÓÀ¸·Î ½Ç½ÃÇÒ ¼ö ÀÖ´Â ¹°Áú »óÈ£ÀÛ¿ë ºÐÀÚ ½Ã½ºÅÛÀÔ´Ï´Ù. µ¶ÀÚÀûÀÎ Grating-coupled Interferometry (GCI, µµÆÄ°ü °£¼·¹ý) ±â¼ú°ú °ß°íÇÑ Microfluidics engineeringÀ» Á¶ÇÕÇØ Á¾·¡ ±â¼ú¿¡¼´Â ÃøÁ¤ÀÌ °ï¶õÇß´ø »ùÇÃÀ» ³ôÀº °¨µµ, °í¼Ó ÃøÁ¤À» ½ÇÇöÇß½À´Ï´Ù.
- 2
ºÐ¼® °¡´É ¹°Áú (ģȵµ, Ä«À̳×ƽ½º)
Crude Sample (Ç÷û ¶Ç´Â Ç÷Àå, Ç×ü, ¹ÙÀÌ·¯½º À¯»ç ÀÔÀÚ, ¹ÙÀÌ·¯½º, ¸®Æ÷Á», ¸· ´Ü¹éÁú), ¼ÒºÐÀÚ/´ÜÆí ¶óÀ̺귯¸®, Å« ºÐÀÚ µî ´Ù¾çÇÑ °Ëü¿¡ ´ëÀÀÇÏ¿© ¿¬±¸¸¦ °¡¼ÓȽÃų ¼ö ÀÖ½À´Ï´Ù.
- 3
°í°¨µµ ÃøÁ¤(ģȵµ, Ä«À̳×ƽ½º)
Rmax 1 pg/mm2 ÀÌÇÏÀÇ ½ÅÈ£µµ ½Å·ÚÇÒ ¼ö ÀÖ´Â °í°¨µµ
Signal to noise 0.01 pg/mm2 ÀÌÇÏÀÇ ½ÅÈ£±îÁö ½Å·Ú °¡´ÉÇÑ °í°¨µµ
ºÐÀÚ·®ºñ 1000:1À» ³Ñ´Â ÀÛ¿ë±â ´Ü¹éÁú (ligand)¿Í ¾à¹° (analyte)¸¦ ºÐ¼® °¡´É - 4
°í¼Ó, ´ë·® ÃøÁ¤
waveRAPID¢ç ºÐ¼®¹æ¹ýÀ¸·Î ºÐ¼® ½Ã°£À» ´ëÆø ´ÜÃà½ÃÅ°°í high throughput testing ½ÇÇö
waveRAPID¢ç ºÐ¼®¹æ¹ýÀº 1ȸ ÁÖÀÔ¸¸À¸·Îµµ °áÇÕ ¹ÝÀÀ (Ä«À̳×ƽ½º) ÃøÁ¤ÀÌ °¡´ÉÇÑ ¹æ¹ýÀÔ´Ï´Ù.
ÀÚ¼¼ÇÑ ³»¿ëÀº ¾Æ·¡ ³»¿ëÀ» Âü°í ºÎŹµå¸³´Ï´Ù.
* ¿¹: 90 ¿©°³ÀÇ ÀúºÐÀÚ ÈÇÕ¹° ºÐ¼®À» 18 ½Ã°£³» ºÐ¼® °¡´É (±âÁ¸ multi-cycle kineticsÀÇ °æ¿ì, 6¹è ÀÌ»óÀÇ ½Ã°£ ¼Ò¿äµÊ)
¡Ø waveRAPID¢ç´Â WAVEdelta ¸ðµ¨¿¡¼¸¸ ÀÛµ¿ÇÕ´Ï´Ù. ¿Ü°üÀº °°À¸¸ç, ÀÚ¼¼ÇÑ ³»¿ëÀº specification¶õÀ» È®ÀÎ ºÎŹµå¸³´Ï´Ù. - 5
»ùÇôç Ĩ È°¿ë ´Ü°¡°¡ ³·°í »ç¿ëÀÌ Æí¸®ÇÑ WAVEchip
- ±³Â÷¿À¿°ÀÌ ¾ø°í ½¬¿î À¯Áö º¸¼ö
- ¸¶ÀÌÅ©·Î ¹ëºê ¾ø´Â µðÀÚÀÎ
- ¾Æ¼¼Åä´ÏÆ®¸±, °í³óµµÀÇ DMSO µîÀÇ ¿ë¸Å¿¡ ´ëÀÀ
- Æø³ÐÀº »ùÇÿ¡ ÀûÀÀÇϴ dzºÎÇÑ ¶óÀξ÷
- Crude sample (Ç÷û ¶Ç´Â Ç÷Àå, Ç×ü, ¹ÙÀÌ·¯½º-À¯»ç ÀÔÀÚ, ¹ÙÀÌ·¯½º, ¸®Æ÷Á», ¸· ´Ü¹éÁú), ÀúºÐÀÚ/´ÜÆí ¶óÀ̺귯¸®, ³À̵µ°¡ ³ôÀº ÈÇÕ¹°, 1000nm±îÁöÀÇ ÀÔÀÚ¿¡ ´ëÀÀ - 6
Æø³ÐÀº ÃøÁ¤ ¹üÀ§: koff =10-5 ~10 s-1
Ä«Æ®¸®Áö ¼³°è¿¡ ÀÇÇÑ 150ms (¹Ð¸®ÃÊ)ÀÇ ÃÊ°í¼Ó ÀüÀÌ ½Ã°£À» ½ÇÇö, ºü¸¥ off-rate ºÐÇØ´ÉÀ» Á¦°ø(KD (Equilibrium constant)ÀÇ ¹üÀ§´Â 1 pM ~ 1 mM ÀÔ´Ï´Ù.)
- 7
Á÷°üÀûÀÎ Á¶ÀÛÀ¸·Î ½±°Ô ½ÇÇè ¼öÇà ¹× °á°ú Çؼ®ÀÌ °¡´ÉÇÑ WAVEcontrol ¼ÒÇÁÆ®¿þ¾î
- 8
Autosampler´Â 48 vial rack, 96/384 well plate µÎ°³¸¦ ä¿ëÇÏ¿© ÃÖ´ë 1~768 °³ »ùÇà žÀç °¡´É
Grating Coupled Interferometry (GCI, ±×·¹ÀÌÆà °áÇÕ °£¼·¹ý) ¿ø¸® ¼³¸í
- Sensing area (1mm2)ÀÇ ³ÐÀº ¹üÀ§¿¡¼ ½ÅÈ£¸¦ ¾òÀº ÈÄ, Reference light¿Í °£¼·À» ÀÏÀ¸ÄÑ À§»óÀÇ º¯È¸¦ ÃøÁ¤ÇÕ´Ï´Ù.
- ¸¸¾à ¼¾¼ Ç¥¸éÀÇ ¹°Áú »óÈ£ ÀÛ¿ëÀÌ ÀϾ¸é, À§»óÀÇ º¯È(yÃà)¸¦ ½Ã°£(xÃà)¿¡ µû¸¥ ½ÅÈ£·Î ³ªÅ¸³¾ ¼ö ÀÖ½À´Ï´Ù.
- ³ôÀº ½ÅÈ£¿Í reference light¿Í °£¼·À» ÅëÇØ high signal to Low noise ratio ¹× ³ôÀº ÇØ»ó´ÉÀ» ½ÇÇöÇß½À´Ï´Ù (noise < 0.01 pg/mm2, Rmax < 1 pg/mm2 (1 pg/mm2= 1RU))
- º» WAVE system¿¡´Â °í°¨µµ ¼º´ÉÀÇ GCI ¼¾¼¸¦ žÀçÇÏ¿© º¸´Ù ½Å·Ú¼ºÀÌ ³ôÀº Ä«À̳×ƽ½º ºÐ¼®ÀÌ °¡´ÉÇÕ´Ï´Ù.
GCI ±â¼ú ¼³¸í ¾Ö´Ï¸ÞÀÌ¼Ç (À¯Æ©ºê µ¿¿µ»ó Ŭ¸¯)
Label-free, Real-time Molecular Interaction analysisÀ̶õ?
Label-free Sample?
Çü±¤À» ºÙÀÎ probe³ª HRPµîÀ» ºÙÀÎ antibodyµîÀÇ ¶óº§À» ºÙÀÌÁö ¾ÊÀº °ÍÀ» ÀǹÌÇÕ´Ï´Ù. Á¾Àü¿¡´Â »ùÇà °¢°¢¿¡ ´ëÇØ taggingÀ» ÇʼöÀûÀ¸·Î ÀÌÇàÇØ¾ß Çß½À´Ï´Ù. º» Á¦Ç°¿¡¼´Â »ùÇà Àüó¸® (Labeling)ÇÏÁö ¾Ê°í ƯÀ̼ºÀ» °¡Áö´Â ¹°Áú¿¡ ´ëÇÑ °áÇÕ ¹ÝÀÀÀ» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Real-time Molecular Interaction analysis?
- »ç¿ëÀÚÀÇ ºÐ¼® ¸ñÀû¿¡ µû¶ó ¾à¹° È帰£ ¹°Áú °áÇÕ ¹ÝÀÀÀ» ¼±º° (screening) ¶Ç´Â Ư¼º ºÐ¼®(characterization)ÀÇ ¸ñÀûÀ¸·Î »ç¿ë °¡´ÉÇÕ´Ï´Ù.
- 1°³ÀÇ target ÀÛ¿ë±â ´Ü¹éÁú (Ligand¶ó°í ºÎ¸§)À» chip¿¡ °íÁ¤½ÃŲ µÚ, ´Ù¼öÀÇ ¾à¹° È帱º (analyte¶ó°í ºÎ¸§)¸¦ ¼øÂ÷ÀûÀ¸·Î Èê·ÁÁÖ¾î °áÇÕ Ä£Èµµ¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù.
- ÀÛ¿ë±â ´Ü¹éÁú°ú ¾à¹° È帰£ °áÇÕ (association), °áÇÕ ÆòÇü»óÅ (equilibrium-state), Çظ® (dissociation) µîÀÇ °áÇÕ »óŸ¦ ½Ç½Ã°£À¸·Î È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
¹°Áú °áÇÕ ¹ÝÀÀ¿¡ µû¸¥ ½Ç½Ã°£ Sensorgram °á°ú
»ç¿ë¿¹½Ã
¿¹¸¦ µé¾î Äڷγª ¹ÙÀÌ·¯½º Ä¡·áÁ¦ °³¹ßÇϱâ À§ÇØ ÀÛ¿ë±â ´Ü¹éÁú (spike protein) ¹ÝÀÀÇÏ´Â ¹°ÁúÀ» ã´Â ¿¬±¸¸¦ ÇÑ´Ù°í °¡Á¤Çغ¸°Ú½À´Ï´Ù. ½ÇÇèÀÚ´Â WAVEchip¿¡ ÀÛ¿ë±â ´Ü¹éÁú (spike protein, ligand)À» °íÁ¤½ÃÄÑÁÝ´Ï´Ù. ±×¸®°í ³ ÈÄ¿¡, ´Ù¼öÀÇ ¾à¹° È帱º (analyte)ÀÎ, ´Ù¼öÀÇ Ãµ¿¬¹°, ´Ù¼öÀÇ chemical compound, ´Ù¼öÀÇ Protein Èĺ¸ ¹°Áú µîÀ» ¼øÂ÷ÀûÀ¸·Î Èê·ÁÁÖ¾ú½À´Ï´Ù. ÀÌ Áß ´Ù¼öÀÇ ¹ÝÀÀÇÏ´Â ¹°ÁúÀ» 1Â÷ ¼±º°ÇÑ µÚ, ÈÄ¼Ó ½ÇÇèÀ» ÅëÇØ ÃÖÁ¾ 1°³ ¹°ÁúÀ» Ä¡·áÁ¦ È帷Π¼±Á¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀÌ·¯ÇÑ ¿¹½Ã¸¦ ÅëÇØ °áÇÕ ¹ÝÀÀÇÏ´Â ¹°ÁúÀ» ½Ç½Ã°£À¸·Î ¼±º°ÇÒ ¼ö ÀÖÀ¸¸ç, ƯÈ÷, ¾ó¸¶³ª ºü¸£°Ô ºÙ´ÂÁö, ´Ê°Ô ¶³¾îÁö´ÂÁö µîÀÇ affinity, kinetics ºÐ¼®À» ¼öÇàÇÏ¿© ¾à¹°ÀÇ ÇÊ¿ä dose, duration time µîÀ» predictionÇÒ ¼ö ÀÖ½À´Ï´Ù.
°í¼Ó ÃøÁ¤À» À§ÇÑ waveRAPID¢ç ºÐ¼®¹ý
- WAVEdelta ¸ðµ¨¿¡¼¸¸ °¡´ÉÇÑ waveRAID¢ç¹æ¹ýÀº ±âÁ¸ multi-cycle traditional kinetics (MCK)¿Í ´Ù¸¥ ȹ±âÀûÀÎ ¹æ¹ýÀÔ´Ï´Ù.
- 1°³ »ùÇôç 1°³ Èñ¼®¾× (higher starting concentration 1°³)¸¸ ÁغñÇÏ¸é µË´Ï´Ù.
- 1°³ »ùÇÃÀ» sample loop·Î »¡¾ÆµéÀÎ ÈÄ, ÀÚµ¿À¸·Î pulse ½Ã°£À» Á¶ÀýÇÏ¿© ³»»ÕÀ¸·Î½á 1ȸ Áß ´Ù³óµµ ºÐ¼®À» ½Ç½ÃÇÏ°í, dissociationÀº 1ȸ ¼öÇàÇϴ ȹ±âÀûÀÎ ¹æ¹ýÀÔ´Ï´Ù.
- 1 ½ÎÀÌŬ¸¸À¸·Î full kinetics (ka, kd, KD, Rmax) ºÐ¼®ÀÌ °¡´ÉÇÑ È¹±âÀûÀÎ ºÐ¼®¹æ¹ýÀÔ´Ï´Ù.
- screening Áß MCK¹æ¹ýÀ¸·Î ¼öÇà ½Ã, 1»ùÇà 1³óµµ·Î ¼öÇàÇϰԵǸç, ÀÌ°æ¿ì full kinetics data¸¦ ¾òÁö ¸øÇÏ°í ´ÜÁö affinity È®Àθ¸ °¡´ÉÇÑ ´ÜÁ¡À» º¸¿ÏÇÑ µ¶Æ¯ÇÑ ¹æ¹ýÀÔ´Ï´Ù.
- Creoptix»çÀÇ Æ¯Çã ¹æ¹ýÀÔ´Ï´Ù.
Regeneration-free kinetics ½ÇÇè°ú ¸¶Âù°¡Áö·Î, ÈÄ¼Ó ºÐ¼® ÆÞ½º°¡ µµÀԵDZâ Àü¿¡ ÀÀ´äÀº ±âÁؼ±À¸·Î µ¹¾Æ°¥ ÇÊ¿ä°¡ ¾ø½À´Ï´Ù. ´ÜÀÏ ³óµµ ÁÖÀÔ ½Ã°£Àº WAVEcontrol ¼ÒÇÁÆ®¿þ¾î¿¡ ÀÇÇØ ÀÚµ¿À¸·Î Á¶Á¤µË´Ï´Ù. À̸¦ ÅëÇØ ´Ü°è Èñ¼®ÀÌ ÇÊ¿ä¾ø°í 1 À£¿¡¼ÀÇ Ä«À̳×ƽ½º ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.
¼Â¾÷ ½Ã°£À» ´ëÆø ´ÜÃàÇÏ°í,»ùÇà 1°³´ç ºÐ¼®À» °¡¼ÓÈÇÏ¿©, º¸´Ù ´õ ¸¹Àº »ùÇÿ¡ ´ëÇØ ºÐ¼® °¡´É¼ö ÀÖ½À´Ï´Ù.
¼ÒÇÁÆ®¿þ¾î WAVEcontrol¢ç
WAVEcontrolÀº À¯¿¬¼º°ú ±â´É¼ºÀ» °áÇÕÇÑ ¿öÅ©Ç÷Π±â¹Ý ¼ÒÇÁÆ®¿þ¾îÀÔ´Ï´Ù. ºÐ¼® ¼³Á¤, µ¥ÀÌÅÍ Æò°¡ºÎÅÍ º¸°í¼ ÀÛ¼º¿¡ À̸£±â±îÁö ¸ðµç ´Ü°è¸¦ Á÷°üÀûÀÎ Á¶ÀÛÀ¸·Î ½±°Ô ¼öÇàÇÒ ¼ö ÀÖ½À´Ï´Ù. ´ÙÀ½ ±â´ÉÀÌ Æ÷ÇԵ˴ϴÙ.
- - ³»ÀåµÈ ¸¶¹ý»ç (wizard) ¹× ÀÌÀü ½ÇÇè¿¡¼ ¼³Á¤ÇÑ »çÀÌŬ (preset)À» È°¿ëÇÏ°í »ç¿ëÀÚ ÁöÁ¤ÇÏ¿© ½Å¼ÓÇÏ°Ô ºÐ¼® ¼³Á¤ °¡´É
- - kinetics fittingÀ» À§ÇÑ ¹Ì¸® Á¤ÀÇµÈ ¸ðµ¨ (1:1 interaction, mass transport, heterogenous ligand, conformational change, bivalent) ¹× user customized evaluation model »ç¿ë °¡´É
- - Traditional fitting (Global fitting)À¸·Î µ¥ÀÌÅÍ Æò°¡ ¶Ç´Â Direct Kinetics µµ±¸¸¦ »ç¿ëÇÑ ÀÚµ¿ ºÐ¼® °¡´É
- - Excel ¹× ±âŸ Çü½ÄÀ¸·Î µ¥ÀÌÅÍ °¡Á®¿À±â ¹× ³»º¸³»±â
- - Open XML Çü½ÄÀÇ ÆÄÀÏÀ» »ç¿ëÇÏ¿© ±âÁ¸ LIMS¿¡ µ¥ÀÌÅÍ ÅëÇÕ (Gene data Screener¿Í ȣȯ °¡´É)
- - ÀÚµ¿ º¸°í ±â´ÉÀ¸·Î »ç¿ëÀÚ ÁöÁ¤ °¡´ÉÇÑ PDF ¶Ç´Â Word Çü½ÄÀ¸·Î °á°ú ³»º¸³»±â
References
Small Molecules/FBDD
Nature Communications, 2021
C. Hong, NJ Byrne, B. Zamlynny, S. Tummala, L. Xiao, JM Shipman, AT Partridge, C. Minnick, MJ Breslin, MT Rudd, et al. ¡°Structures of active -state orexin receptor 2 rationalize peptide and small-molecule agonist recognition and receptor activation.¡± DOI: 10.1038/s41467-021-21087-6
Small Molecules/FBDD
RSC Advances, 2021
EA Fitz Cederfelt, M. Abramsson, H. Ludviksdottir, JE van Muijlwijk-Koezen, IJP de Esch, D. Dobritzsch, T. Young, H. Danielson. ¡°Discovery of fragments inducing conformational effects in dynamic proteins using a second-harmon .¡± DOI: 10.1039/D0RA09844B
Small Molecules/FBDD
Scientific Reports, 2020
H. Jankovics, B. Kovacs, A. Saftics, T. Gerecsei, E. Tóth, I. Szekacs, F. Vonderviszt, R. Horvath. ¡°Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers.¡± DOI : 10.1038/s41598-020-79226-w
Plant Sciences, Peptides
PNAS, 2020
AD Crook, AC Willoughby, O. Hazak, S. Okuda, KR VanD NM Clark, R. Sozzani, M. Hothorn, CS Hardtke, ZL Nimchuk. ¡°BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots.¡± DOI : 10.1073/pnas.2018565117
COVID-19 Research
bioRxiv, 2020
JD Walter, CAJ Hutter, AA Garaeva, M. Scherer, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, JC Earp, P. Egloff, M. Sorgenfrei, L. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. Zimmer, DJ Slotboom, C. Paulino, P. Plattet, MA Seeger. ¡°Highly potent bispecific sybodies neutralize SARS-CoV-2.¡± DOI : 10.1101/2020.11.10.376822
Biologics Development, COVID-19 Research
bioRxiv, 2020
JD Walter, CAJ Hutter, I. Zimmermann, M. Wyss, P. Egloff, M. Sorgenfrei, LM Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah , P. Plattet, MA Seeger. ¡°Sybodies targeting the SARS-CoV-2 receptor-binding domain.¡± DOI : 10.1101/2020.04.16.045419
Protein-Protein Interactions
Analyst, 2019
B. Peter, A. Saftics, B. Kovacs, S. Kurunczi, R. Horvath. ¡°Oxidation increases the binding of EGCG to serum albumin revealed by kinetic data from label-free optical biosensor with reference channel.¡± DOI : 10.1037/ C9AN0
Plant Sciences, Peptides
The EMBO Journal, 2019
K. Lau, R. Podolec, R. Chappuis, R. Ulm, M. Hothorn. ¡°Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP peptide motifs.¡± DOI: 10.15252/embj.2019102140
Biologics Development
Journal of Molecular Biology, 2019
F. Andres, L. Iamele, T. Meyer, JC Stüber, F. Kast, E. Gherardi, HH Niemann, A. Plückthun. ¡°Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking.¡± DOI: 10.1016/j.jmb.2019.03.024
Plant Sciences
Nature Plants, 2018
U. Hohmann, J. Nicolet, A. Moretti, LA Hothorn, M. Hothorn, ¡°The SERK3 elongated allele defines BIR ectodomains in brassinosteroid signalling.¡± DOI: 10.1038/s41477-018-0150-9
Small Molecules/FBDD
E-Life, 2018
W. Pitsawong, V. Buosi, R. Otten, RV Agafon Kern, S. Kutter, G. Kern, RAP Pádua, X. Meniche, D. Kern. ¡°Dynamics of human protein kinase Aurora A linked to drug selectivity.¡± DOI: 10.7554/eLife.36656
¡ØÀÌ ¿Ü¿¡µµ ´Ù¼öÀÇ ³í¹®ÀÌ ÀÖ½À´Ï´Ù. ÀÚ¼¼ÇÑ ³»¿ëÀº ¹®ÀÇÇϽñ⠹ٶø´Ï´Ù.
Specification
Creoptix WAVE
General
- Noise (RMS)
- <0.01 pg/mm2 @ 1 Hz
- Drift
- <0.3 pg/mm2/min
- Readout Frequency
- 1 Hz, 10 Hz or 40 Hz
- Association Const. Range
- ka = 103 – 5x107 M-1 s–1 (small molecules)
ka = 103 – 3x109 M-1 s–1 (large molecules)
- Dissociation Const. Range
- kd = 10–5 – 10 s–1
- Analysis Temperature Range
- 15¡ÆC – 40¡ÆC
- Molecular Weight Limit
- No lower limit
- waveRAPID¢ç Functionality
- No
Fluidics
- Flow Channels / Path
- 2, parallel
- Channel Referencing
- 1-4 and 4-1 or 2-3 and 3-2
- Flow Cells
- Sealed, disposable, integrated into disposable WAVEchip
- Flow Rate
- 1 – 400 µl/min
- Crude Sample Robustness
- Yes
Sample Handling
- Sample Capacity
- 2x microtiter plates
(96 or 384 well, standard or deep well) or vial racks (48 positions of 1.5ml)
- Buffer
- 1 buffer
- Degasser
- Built-in
- Injection Volume
- < 450 µl, 100 µl typical
- Sample Volume Required
- Injection volume plus 15-50 µl (application dependent)
- Sample Storage Temperature
- Ambient or 4¡ÆC – 20¡ÆC regulated
- Sample Recovery
- Yes
- Automation
- 120h of unattended operation
Data Treatment
- Information Provided
- Kinetic and affnity data (ka, kd, KD)
- Graphs
- Real-time curves, multiple curve overlays, fit, report point plots
- Data Extraction
- Curves, ka, kd, KD tables, graphs, reports
- Data Analysis
- Fully automated data evaluation
- Kinetic Models
- Predefined models including 1:1 interaction, mass transport, heterogenous ligand, conformational change and bivalent
- Direct Kinetics
- Yes
Creoptix WAVEdelta
General
- Noise (RMS)
- <0.01 pg/mm2 @ 1 Hz
- Drift
- <0.3 pg/mm2/min
- Readout Frequency
- 1 Hz, 10 Hz or 40 Hz
- Association Const. Range
- ka = 103 – 5x107 M-1 s–1 (small molecules)
ka = 103 – 3x109 M-1 s–1 (large molecules)
- Dissociation Const. Range
- kd = 10–5 – 10 s–1
- Analysis Temperature Range
- 4¡ÆC – 45¡ÆC (max 20¡ÆC below ambient)
- Molecular Weight Limit
- No lower limit
- waveRAPID¢ç Functionality
- Yes
Fluidics
- Flow Channels / Path
- 4, parallel
- Channel Referencing
- Any combination of the 4 channels
- Flow Cells
- Sealed, disposable, integrated into disposable WAVEchip
- Flow Rate
- 1 – 400 µl/min
- Crude Sample Robustness
- Yes
Sample Handling
- Sample Capacity
- 2x microtiter plates
(96 or 384 well, standard or deep well) or vial racks (48 positions of 1.5ml)
- Buffer
- Automatic switching between 4 buffers
- Degasser
- Built-in
- Injection Volume
- < 450 µl, 100 µl typical
- Sample Volume Required
- Injection volume plus 15-50 µl (application dependent)
- Sample Storage Temperature
- Ambient or 4¡ÆC – 20¡ÆC regulated
- Sample Recovery
- Yes
- Automation
- 120h of unattended operation
Data Treatment
- Information Provided
- Kinetic and affnity data (ka, kd, KD)
- Graphs
- Real-time curves, multiple curve overlays, fit, report point plots
- Data Extraction
- Curves, ka, kd, KD tables, graphs, reports
- Data Analysis
- Fully automated data evaluation
- Kinetic Models
- Predefined models including 1:1 interaction, mass transport, heterogenous ligand, conformational change and bivalent
- Direct Kinetics
- Yes
Product Information
Cat No. | Ç°¸í | ±Ô°Ý | Á¦Á¶»ç | Á¦Ç°Á¤º¸ |
---|---|---|---|---|
WAVE-2h | WAVEsystem | - | Creoptix |
Label-free, Real-time, Automated - Molecular Interaction Analyzer - Surface-based Microfluidic pump system - 2 Channel - GCI biosensor equipped - No-Clog Microfluidics consumables (WAVEchip) - 1-Click operation and evaluation software: WAVEcontrol |
WAVEdelta-4ch | WAVEdelta | - | Creoptix |
Label-free, Real-time, Automated - Molecular Interaction Analyzer - Surface-based Microfluidic pump system - 4 Channel - GCI biosensor equipped - No-Clog Microfluidics consumables (WAVEchip) - 1-Click operation and evaluation software: WAVEcontrol - waveRAPID analysis Included (Prepare 1 dilute per 1 sample, Inject once, and Obtain 1 full kinetic data set) |
Releated Product
WAVEchip¢ç ¶óÀξ÷
´Ù¾çÇÑ »ùÇÃÀ» ÀÌ¿ëÇÑ ¾îÇø®ÄÉÀ̼ÇÀ» À§ÇØ ´Ù¾çÇÑ Ç¥¸éÀÇ ¼¾¼ ĨÀ» ÁغñÇÏ°í ÀÖ½À´Ï´Ù.
PC*: Polycarboxylate ÀçÁú
DX*: Dextran ÀçÁú